In this paper, we demonstrate the existence and localization of fucosyl-containing O-glycoforms of nucleolin in cultured bovine endothelial cells (CVEC) and malignant cultured human A431 cells. The tool for this discovery was an antibody raised against gp273, a glycoprotein ligand for the sperm鈥...
After washing, cells were incubated with PE-labeled secondary antibody in binding buffer for 15 min at room temperature and then in Fix & Perm Reagent A (Fixation Medium) according to the manufacturer's instructions (Caltag Laboratories, Burlingame, CA). Cells were washed and divided into two ...
Relatively modest changes, such as the extent or position of O-acetylation orO-methylation, could easily account for some of the alterations in antibody reactivity. Recently, Carlson et al.(47) surveyed some of the chemical changes occurring in R. leguminosarum LPS isolated from normally cultured ...