然而,在类风湿性关节炎(Rheumatoid arthritis,RA)等炎性关节炎中,破骨细胞分化和活性的增强打破了这种平衡,导致进行性骨丢失。O-GlcNAcylation是一种翻译后修饰,是将单个O-连接的β-D-N-乙酰氨基葡萄糖(O-GlcNAc)残基连接到靶蛋白的...
HEK 293T细胞中内源性SRPK2被ShRNA面向的SRPK2 3’ UTR消耗, 然后与cDNA的编码WT 或SRPK2中O-GlcNAcylation缺陷突变体进行稳定的重组(4A或3A或T237A)。用免疫染色法检测SRPK2细胞的定位。 HEK 293T细胞中内源性SRPK2被ShRNA面向的SRPK2 3’ UTR消耗, 然后与cDNA的编码WT 或SRPK2中O-GlcNAcylation缺陷突变...
以GST-SRPK2(氨基酸[aa]454-521)为底物的体外O-GlcNAcylation试验。 用指定的质粒转染HEK 293T细胞。用anti-SRPK2抗体免疫沉淀细胞裂解物,用指定的抗体测定SRPK2 O-GlcNAcylation。 用指定的siRNA和质粒转染HEK293T细胞,用指定的抗体通过IP和WB检测SRPK2 O-Glc...
by dysregulating C1GalT1 and ST6GalNAc-II enzymes. 9. Chen L, 2023, Direct stimulation of de novo nucleotide synthesis by O-GlcNAcylation. 10. Shi J, 2021, Comprehensive analysis of O-glycosylation of amyloid precursor protein (APP) using targeted and multi-fragmentation MS strategy.
2. Yang, X. and K. Qian, Protein O-GlcNAcylation: emerging mechanisms and functions. Nat Rev Mol Cell Biol, 2017. 18(7): p. 452-465.3. Howerton, C.L., et al., O-GlcNAc transferase (OGT) as a placental biomarker of maternal stress and reprogramming of CNS gene transcription in ...
9. Chen L, 2023, Direct stimulation of de novo nucleotide synthesis by O-GlcNAcylation. 10. Shi J, 2021, Comprehensive analysis of O-glycosylation of amyloid precursor protein (APP) using targeted and multi-fragmentation MS strategy. 景杰生物作为修饰组学领域的领跑者,拥有多种修饰抗体和修饰组学质...
2. Yang, X. and K. Qian, Protein O-GlcNAcylation: emerging mechanisms and functions.Nat Rev Mol Cell Biol, 2017. 18(7): p. 452-465. 3. Howerton, C.L., et al., O-GlcNAc transferase (OGT) as a placental biomarker of maternal stress and reprogramming of CNS gene transcription in ...
Quantitative chemoproteomics reveals O-GlcNAcylation of cystathionine γ-lyase (CSE) represses trophoblast syncytialization 3. O-GlcNAc+磷酸化：动态调控春化作用介导小麦开花 2019, IF:6.035 The Protein Modifications of O-GlcNAcylation and Phosphorylation Mediate Vernalization Response for- Flowering in Winter ...
9. Chen L,et al.2023, Direct stimulation of de novo nucleotide synthesis by O-GlcNAcylation.Nat Chem Biol. 10. Shi J,et al.2021, Comprehensive analysis of O-glycosylation of amyloid precursor protein (APP) using targeted and multi-fragmentation MS strategy.Biochim Biophys Acta Gen Subj. ...