c, H1299 cells were transfected with the indicated siRNAs and cultured with 1 μCi of14C-glucose. The de novo synthesized14C-RNA (b) or14C-DNA (c) was measured by liquid scintillation counting. Immunoblot wit
E., Ryazankina, O. I., Shchelkunov, S. N., Koonin, E. V. & Chernos, V. I. (1993). Nucleotide sequence of XhoI O fragment of ectromelia virus DNA reveals significant differences from vaccinia virus. Virus Res 30, 73-88.
El panel consideró que no es prudente continuar la terapia biológica con rituximab durante procesos infecciosos activos, entre ellos COVID-19, por lo que se recomienda que los pacientes con enfermedades reumáticas con diagnóstico de COVID-19 deben suspender la terapia con rituximab. Tocilizumab...
Role of TET enzymes in DNA methylation, development, and cancer. Genes Dev. 30, 733–750 (2016). Article CAS PubMed PubMed Central Google Scholar Shi, F. T. et al. Ten-eleven translocation 1 (Tet1) is regulated by O-linked N-acetylglucosamine transferase (Ogt) for target gene ...
Candida glycerinogenesWL2002-5 has a modest sugar tolerance and an extremely high glycerol productivity.Agrobacterium tumefacienscan transfer part of its Ti plasmid, the T-DNA, into the nuclear genome of a wide variety of host cells. In this study, we constructed the plasmid pZR and transferred...
editing tools allow genome engineering while avoiding the need for homologous recombination events, the introduction of donor DNA and DNA double-strand breaks [163,320]. A useful application for base editing tools is the introduction of premature stop codons into genes to disrupt the gene function....
E - The same protein samples separated by SDS-PAGE (4-20% gradient gel) after staining with Coomassie Brilliant Blue R-250; Abbreviations used: C - control plant; 1,2 - putative transgenic plants; M- markers: Promega 1 kb DNA ladder (B, C), BioRad Kaleidoscope prestained standards (D...
The DNA-binding sites of Runx2 have been identified in major osteoblast-specific genes, including the genes that encode type I collagen(Col1a1),Runx2itself, and Runx2 induced the expression of these genes or activated their promoters in vitro.226It is puzzling that although Runx2 strongly indu...
ATM-phosphorylated SPOP contributes to 53BP1 exclusion from chromatin during DNA replication. Sci Adv. 2021;7:eabd9208. de Queiroz RM, Carvalho E, Dias WB. O-GlcNAcylation: the sweet side of the cancer. Front Oncol. 2014;4:132. Article PubMed PubMed Central Google Scholar Hu J, Gao ...
The RNA was DNAse-treated (DNase I-RNase-Free, Ambion) to remove any contaminating DNA; 200 ng of total RNA was reverse-transcribed with oligo dT primers using the High Capacity cDNA RT Kit (Applied Biosystems) in a 20-μl cDNA reaction, as specified by the manufacturer. For ...