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Samples were washed two times with PBS and resuspended in 500 µL of PBS/2 mM of EDTA. Additionally, trypan blue quenching was used to exclude the surface fluorescence signals of not completely internalized siRNA complexes. Results were compared to those obtained with cells that did not ...
UV absorption spectra of CO-bound artificial self-sufficient P450 proteins after sodium dithionite reduction were measured by Cary 100 UV-Vis spectrometry (Agilent Technologies, CA, USA) by wavelength scan from 400 to 500 nm. The concentration of P450 was measured by CO-binding affinity using an...