Finally, the pellet of bee tissue was subjected glycogen analysis (hot anthrone). For total carbohydrates, the homogenized abdomens were diluted in water so that the values fell within the quantifiable range of the test. Fifty µL of the diluted samples was added to 450 µL chloroform:...
Thus, understanding how nutrition impacts the mecha- nisms underlying insulin resistance requires a quantitative analysis and description of a multiscale, highly coupled regulatory network that includes thousands of components, ranging over subcellular to whole body levels of organization and ...
Previous insights into the nutritional strategies of coral reef fishes have come from the analysis of the products of intestinal microorganisms, i.e. short chain fatty acids (SCFA). These compounds are assimilated and used as a metabolic substrate by the fishes (Clements 1997, Mountfort et al. ...
Because changes in serum LPS levels, intestinal eCB tone, intestinal immune cells and intestinal MyD88 deletion have been previously associated with specific gut microbiota composition, we performed a deep gut microbiota analysis using high-throughput sequencing. Consistent with previous studies6,32,39,...
Fatty acid analysis. The ratios of grain fatty acids were determined by hydrolysis and fatty acid methyl ester formation modified from the procedure of Hartman and L ago39, and analysis by gas chromatography mass spectrometry (GC–MS) in triplicate. Briefly, 60 mg of whole wheat ...
For analysis, a Thermo Q Exactive Orbitrap MS (Thermo Fisher Scientific, Massachusetts, NC, USA) with electrospray ionization probe interface was used, in positive and negative-ion mode. Full scan was carried out upon conditions described in Zengin et al. [88]. Thermo Scientific Xcalibur 3.1 ...
Using a JASCO 6200 (USA) outfitted with a Ge ATR, Fourier-transform infrared (FT-IR) analysis was carried out. All investigations were carried out between 400 and 4000 cm−1 at a resolution of 2 cm−1, and the software Spectra Manager v2 was used to handle the data. ...
Analysis of variance (ANOVA) was performed to evaluate the significance of the model terms and interactions between the factors at a 95% confidence level (p≤ 0.05). The software’s optimization module was then used to determine the optimal conditions for maximizing WL and SG. The optimization ...
A meta-analysis [29] of four small randomized controlled trials (n = 107) [30–33] found that compared with placebo, omega-3 fatty acid supplementation improved social withdrawal (p < 0.02) and restricted interests and behaviors (p = 0.05), but did not have a significant effect on ...
The data were analyzed using analysis of variance (F test) and Tukey’s test at 5% probability to compare the averages of the N applications, which were adjusted according to the polynomial regression for the glyphosate doses using the SAS system (SAS Institute Inc., Cary, NC, USA). ...