The base unit of chromatin structure is the nucleosome, composed of ~147bp DNA wrapped around a histone octamer1. Nucleosomes are the preferred, physiological substrate of many chromatin interacting proteins, and are required to study numerous high-value therapeutic targets, including histone modifying...
In the nucleus, biological processes are driven by proteins that diffuse through and bind to a meshwork of nucleic acid polymers. To better understand this interplay, we present an imaging platform to simultaneously visualize single protein dynamics toge
Chromatin is composed of core nucleosome particles, each containing approximately 147 bp of double-stranded DNA wrapped around a histone octamer [1]. Nucleosomes restrict the accessibility of proteins to the DNA, thereby influencing DNA transcription, replication, recombination and repair [2–4]. Nucl...
Nucleosomes are DNA鈥損rotein complexes composed of histone proteins that form the basis of eukaryotic chromatin. The nucleosome was a key innovation during eukaryotic evolution, but its origin from histone homologues in Archaea remains unclear. Viral histone repeats, consisting of multiple histone ...
a pair of h2a/h2b dimers (red/pink). dna is held to these core “strong sites” while (d) the outer two half wraps of dna are more weakly organized by contacts with histone coils and tails. (e) heterodimeric full hfact is composed of subunits spt16 (forest) and ssrp1 (green), ...
There is currently a lack of insight into the genome-wide nucleosomal organization of genomes from organisms that are evolutionarily far removed from the Unikonta supergroup of eukaryotes, yet encode conventional nucleosomes composed of a conserved octamer of histones. In this regard,T. bruceirepresent...
In order to develop a technique for the detection of autoreactive B cells by flow cytometry in SLE, we chose the nucleosome – the basic unit of chromatin – as an autoantigen. Nucleosome is composed of 146 DNA base pairs wrapped around two copies of histones H2A, H2B, H3 and H4 (the ...
Nucleosome, the fundamental unit of chromatin, is histone octamer composed of dimers of each histone H2A, H2B, H3, and H4. Histones are the key epigenetic ... A Patankar,P Parte - Springer Singapore 被引量: 5发表: 2017年 Histone removal in sperm protects paternal chromosomes from premature ...
We have previously experienced difficulty assembling nucleosomes on a DNA sequence composed of a 147-bp, 601-core sequence with 45- and 29-bp terminal extensions (45N29) [8], [31]. At a 1:1 molar ratio of octamer to DNA, hexasomes had a propensity to form, as noted by others using...
Figure 1: Chromosomes are composed of DNA tightly-wound around histones. Chromosomal DNA is packaged inside microscopic nuclei with the help of histones. These are positively-charged proteins that strongly adhere to negatively-charged DNA and form complexes called nucleosomes. Each nuclesome is compose...