9.3.1 Nucleic acid sample preparation and enrichment Polymerase chain reaction (PCR) is an in vitro enzymatic multiplication process for defined strands of DNA based on the choice of specific primers. PCR has become the most common procedure to amplify nucleic acids. For example, in reverse trans...
Also, the overexpression of LuxR markedly affected the process of nucleic acid metabolism. For example, the overexpression of LuxR in Gluconacetobacter xylinus caused both the purine and pyrimidine contents to be lower in the mutant than in the wild type, further blocking the formation of nucleic ...
The question of whether community nucleic acid testing contributes to an increase in infections within residential compounds has not been definitively answered. Shanghai, one of the largest cities in China, conducted city-wide community testing during its lockdown from late March to May 2022. This ...
Popular typesASOsiRNAmRNAAptamerAdvantagesFor its fast and intuitive design of base sequences, the development of nucleic acid drugs applies simple materials, convenient preparation processes and affo
These benchtop instruments have a small footprint and combine the convenience of quick real-time nucleic acid analysis and high-quality visualization. The E-Gel electrophoresis systems are available in both high- and standard-throughput capab...
1.1. Nucleic acid delivery In terms of delivery, therapeutic nucleic acids must be transported to their target sites (nucleus for pDNA or RISC in the cytoplasm for siRNA) before producing their biological effects. A delivery system must overcome a series of extracellular and intracellular barriers ...
Nucleic acid is the main material for storing, copying, and transmitting genetic information. Gene sequencing is of great significance in DNA damage research, gene therapy, mutation analysis, bacterial infection, drug development, and clinical diagnosis.
In this respect, nucleic acid therapies targeting microRNA (miRNA) may be attractive because distinct miRNA expression patterns have been found in patients with ischemic stroke with both up- and downregulation of miRNAs as assessed in the blood [140], and also because miRNAs have been implicated ...
For example, fractionation and isolation of pure alkaloids can be achieved using the acid-base fractionation technique followed by CC or HPLC purification processes as outlined in Fig. 16.5. In order to prevent decomposition or the formation of artifacts during separation processes, the alkaloids...
The INNO-LIPAMycobacteriakit (Innogenetics, Ghent, Belgium), which is mainly available in Europe, is an example of probe hybridization coupled withnucleic acid amplification. In this assay, theinternal transcribed spacer(ITS) of 16S-23S rRNA is amplified bypolymerasechain reaction (PCR) first. Then...