nuclear run-on experiment 核试运行 拼音 双语对照 nuclear run-on experiment 英 [ˈnju:kliə ˈrʌnɔn iksˈperimənt] 美 [ˈnukliɚ ˈrʌnˌɑn ɪkˈspɛrəmənt]词典 [医]核连缀...
Nuclei Run-on是一种用于检测细胞内正在转录的mRNA的技术。其原理是通过迅速冷冻细胞核，使正在转录的mRNA复合体处于冻结状态，随后加入ATP、GTP、CTP和32P-UTP，重新启动并终止mRNA的转录，从而标记冻结时正在转录的mRNA。提取mRNA后，通过cDNA探针和对照cDNA标记尼龙膜检测目标mRNA。细胞核的提取过程包括：...
Nuclear Run(改进的实验方法)Nuclear Run-on using b M Re-suspended in 35 ml of extraction buffer (0.25M sucrose, 10mM Tris-HCl pH 6.5, 10 mM NaCl, 5mM EDTA, 0.15 mM spermine, 0.5 mM spermidine, 20mM β- mercaptolethanol, 1% Triton X-100 and 2 X Roche complete protease inhibitors). ...
我想问一下关于nuclear run-on assay的原理以及其与Northern blot的比较,核连缀实验, 答案 Northern印迹杂交(Northern blot).原理:将RNA固定于尼龙膜上以DNA探针识别待检mRNA这是一种将RNA从琼脂糖凝胶中转印到硝酸纤维素膜上的方法.DNA印迹技术由Southern于1975年创建,称为Southern印迹技术,RNA印迹技术...相关推荐...
NuclearRun-OnAssay StephenT.Smale INTRODUCTION Thenuclearrun-onassaywasdevelopedasamethodforestablishingthatthetranscriptioninitiation ratecontributestotheregulatedexpressionofmammaliangenes.Thedifferencebetweenmonitor- inggeneexpressionbythenuclearrun-onassayversusmostotherassaysisthatthenuclearrun-on assayprovidesameasur...
nuclear-run-on-experiment网络核连缀实验 网络释义 1. 核连缀实验 分子生物学与生物工程词汇对... ... nuclear run-on experiment 核连缀(转录)实验 nuclear safeguards technique 核保障监督技术 ... www.foodmate.net|基于1 个网页© 2024 Microsoft 隐私声明和 Cookie 法律声明 广告 帮助 反馈...
Nuclei Run-on 原理：迅速冷冻，使细胞核内正在转录的mRNA复合体处于冻结状态 加入ATP/GTP/CTP/32P-UTP，重新恢复mRNA的转录并使之结束（标记冻结时正在转录的mRNA)，提取mRNA 以cDNA探针及对照cDNA标记尼龙膜后检测目标mRNA 2、操作过程：细胞核的提取 处理细胞（培养于10厘米培养皿）吸去培养液，迅速...
Nuclear run on transcription assay NUCLEAR RUN-ON TRANSCRIPTION Reagents:1.50X Denhardt’s solution: 1% Ficoll, 1% polyvinylpyrrolidone, and 1% bovine serum albumin (BSA). Filter sterilize.2.Dnase I 3.Hybridization buffer: 50% formamide, 6X SSC, 10X Denhardt’s solution, 0.2% SDS 4....
Nuclear “run-on” (or “run-off”) transcription assays have been used to obtain quantitative information about the relative rates of transcription of different genes in nuclei isolated from a particular tissue or organ. This information can then be used in analyzing the factors that control the...
Measured by nuclear run-on assays, transcription rates of the receptor gene were unaffected in cells challenged with isoproterenol alone. Dexamethasone, in contrast, stimulated a 4-fold increase in beta 2-adrenergic receptor gene ... JR Hadcock,HY Wang,CC Malbon - 《Journal of Biological Chemistr...