Nuclear dense fine speckled (DFS) pattern, a competent-level pattern evaluated by the ICAP previously (AC-02),2 is frequently discussed in the current recommendation for it being the pattern best associated with apparently healthy individuals, or negatively correlating with systemic autoimmune rheumatic...
Recognition of the dense fine speckled (DFS) pattern remains challenging: results from an international internet-based survey Article Open access 09 July 2016 Keymessages We report on an international survey on Antinuclear Antibody (ANA) indirect immunofluorescence (IIF) pattern reporting. Both clinicia...
(ANA) HomogeneousPattern 6 Anti-NuclearAntibody(ANA) RimPattern CLS420ClinicalImmunology&MolecularDiagnostics–LectureSeries Immunity&AutoimmuneDiseases2 7 Anti-NuclearAntibody(ANA) SpeckledPattern 8 Anti-NuclearAntibody(ANA) NucleolarPattern 9 Anti-NuclearAntibody(ANA) CentromerePattern Thecentromerepatternhas...
The most common immunofluorescence pattern was a fine dense speckled pattern. In the ANA-positive patients with malignant lesions, seven had positivity for ENA profile (three for anti-RNP and anti-Sm, one for just anti-RNP, two for anti-Ro and anti-La e two for just anti-La). It was ...
Homogenous pattern was positive in 25(71.4%), 5(14.3%) fine speckled, 5(14.3%) centromere, and 1(2.8%) presented nuclear dots. The most frequent titer observed was 1/320 in 14(40%) individuals.Conclusion: The homogenous pattern was frequently observed patterns ANA positive subjects. There ...
Basu A,Woods-Buraham L,Ortiz G,et al.Specificity of antinuclear autoantibodies recognizing the dense fine speckled nuclear pattern:Preferential targeting of DFS70/LEDGFp75 over its interacting partner MeCP2[J].Clin Immunol,2015,161(2):241-50....
Results In addition to the historically described reactivities, 6/12 sera demonstrated novel reactivities or fine specificities: CDC1 (IIF ANA, homogeneous/rim pattern; anti-double stranded DNA (dsDNA): SmBB', RNP-C; CDC2 (IIF ANA, speckled pattern; anti-SS-B/La): SS-A (Ro60), TRIM21...
AuTM-treated A.TL mice showed anti-nuclear antibodies (ANA) with homogenous and/or fine speckled staining patterns and serum autoantibodies to ds-DNA, chromatin, histones, and ribonucleoproteins (RNP). Female A.TL mice showed a stronger immune response than males, as well as an increase of B...
We here describe the approach taken to unambiguously identify the C1/C2 proteins as ANA targets in the sera of some patients. To determine the fine specificity of sera containing an unusual speckled ANA-staining pattern using a combination of 2D gel electrophoresis and MS. Patient sera were ...
The sensitivity was 97.1% for speckled pattern, 91.4% for homogenous pattern, 97.1% for nucleolar pattern, 97.1% for centromere pattern, 97.2% for cytoplastmic pattern, and 100% for both dense fine speckled and mitochondrial patterns. The specificity was also good, ranged between 97.1% and 100...