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energy was set to 35%. ProteoWizard msConvert and Mascot (Matrix Science, London, UK; version 2.4.1) were respectively used to extract and analyze all MS/MS ion spectra. Mascot was set up to search the uniprot_human database (88,625 entries) based on the assumption that the digestion ...
2.7. Confocal microscopy and Fluorescence Resonance Energy Transfer (FRET) assay For FRET assay, HeLa cells were transfected with plasmids expressing ECFP-Nrf2zip (2 μg), and EYFP-MafG or EYFP-MafG mutants (1 μg) in glass bottom dishes (MatTek, Ashland, MA). Twenty four hours after trans...