The effective gene number (the number of genes that can be inherited) of mitochondrial DNA (mtDNA) is one-fourth of that of nuclear DNA (ncDNA) in idealized populations. Therefore, mtDNA haplotype diversity (h) is predicted to be lower than ncDNA heterozygosity (H-E) because of the ...
But, some proteins, which are encoded by nuclear DNA are imported from the cytosol. Nuclear DNA (nDNA) is composed of several linear chromosomes, which encodes almost all the proteins required by the cell. Mitochondrial DNA is short compared to the nuclear DNA. The main difference between ...
(f) qPCR measurements of mtDNA levels normalized to nuclear DNA content (mito target = MT-TL1, nuclear target = B2M; n = 4 replicates from 2 independent experiments, two-tailed t-test, data are means +/− SD). (g) Western blot analysis of OXPHOS subunits in NT, PREPL KO, KO...
Recently, Mok et al.3demonstrated that the interbacterial deaminase toxin DddAtoxderived fromBurkholderia cenocepaciacan be split and fused to TALE arrays and a uracil glycosylase inhibitor (UGI) to create DdCBEs, which catalyze C-to-T base conversions in nuclear DNA and mitochondrial DNA (mtDNA)...
Mitochondria serve as the cellular powerhouse, and their distinct DNA makes them a prospective target for gene editing to treat genetic disorders. However, the impact of genome editing on mitochondrial DNA (mtDNA) stability remains a mystery. Our study reveals previously unknown risks of genome editi...
To evaluate the applicability of plasma ccf nDNA and mtDNA as a biomarker for distinguishing between the three study-groups we performed ROC (Receiver Operating Characteristic) curve analysis. We also compared the levels of both species in the cancer group with clinicopathological parameters. Results ...
线粒体疾病是由核DNA(nuclear DNA,nDNA)或线粒体DNA(mitochondrial DNA,mtDNA)突变引起的一组复杂的先天性代谢缺陷,线粒体病在儿童中的总体发病率达1/2000左右,成年人患病率约1/4300,是最常见的遗传代谢病之一。 线粒病致死率及致残率很高。致病性线粒体DNA变异的人群携带率约为1/200,其中m.3243A>G突变的...
Here we report that Lig3 is essential for mtDNA integrity but dispensable for nuclear DNA repair. Inactivation of Lig3 in the mouse nervous system resulted in mtDNA loss leading to profound mitochondrial dysfunction, disruption of cellular homeostasis and incapacitating ataxia. Similarly, inactivation ...
A single cell contains up to several thousand copies of mitochondrial DNA (mtDNA) opposed to two copies of nuclear DNA (nDNA)22. Hence, examination of cell-free mitochondrial DNA (cf-mtDNA) theoretically could offer a higher level of sensitivity than analyzing cell-free nuclear DNA (cf-nDNA...
, suppressing their expression within the nuclear genome22. Concatenated NUMTs can mimic the paternal transmission of mtDNA, generating a mixed haplotype resembling mtDNA heteroplasmy5. Here we show they increase the likelihood of detecting mixed alleles resembling heteroplasmy (when compared with the ...