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The pro-cell death Bcl-2 family member, BNIP3, is localized to the nucleus of human glial cells: implications for glio- blastoma multiforme tumor cell survival under hypox- ia. Int J Cancer 2006;118:1660–1669. 22 Kamer I, Sarig R, Zaltsman Y, et al. Proapoptotic BID is an ATM ...
The 446 loci retrieved were identified and classified in plastid, mitochondrial and nuclear genome datasets by conducting automatic BLAST searches in NCBI GenBank (https://blast.ncbi.nlm.nih.gov) with an in-house designed script (https://github.com/dickgroenenberg/Bogarin_Anchored_Phylogenomics) an...
the nuclei were broken by sonification on ice in extraction buffer containing 0.7 M sucrose, 0.1 M KCl, 0.5 M Tris-HCl pH 7.5, 0.5 M EDTA, 1 mM PMSF, and 5 mM β-mercaptoethanol. Then an equal volume of Tris-HCl pH7.5-saturated phenol was added...
Database searches using the BLASTX program revealed that Ha-Ntf2 has 80, 75, 68 and 68% identity to Apis mellifera Ntf2, Aedes aegypti Ntf2, Maconellicoccus hirsutus Ntf2, and Drosophila melanogaster Ntf2, respectively [see Additional file 3, A]. The sequence identity of Ha-Ran with oth...
M. 2013. Nuclear fibro- blast growth factor 2 (FGF2) isoforms inhibit bone marrow stromal cell mineralization through FGF23/FGFR/MAPK in vitro. J. Bone Miner. Res. 28, 35-45.Xiao L, Esliger A, Hurley MM (2013) Nuclear fibroblast growth factor 2 (FGF2) isoforms inhibit bone marrow ...
LGP plasmids with right inserts of Gli1gRNAs are then co- transfected with pLenti-Cas9-Blast, pCMV.Dr8.2 and pCMV- VSV.G with ratio of 10 : 10 : 10 : 1 into HEK293T cells with B70% confluency by Lipofectamine2000 (Carlsbad, CA, USA) in six-well plates. Supernatants are collected ...
Orthologous sequences of the primate NONO gene were retrieved using UCSC Blat and NCBI Blastn with the human sequence as the query. Sequences annotated as non-coding (XR/NR identification) were discarded. We confirmed the conserved synteny of the selected orthologous genes using UCSC and NCBI. ...
The 4T1 Padi4WT or Padi4KO tumor cells were transduced with an iCasp9 vector that was made using a Gibson (NEB E5510S) kit and the iCasp9 gene from the EF1α-iCasp9-hΔCD19 vector44 was cloned into a pLV-EF1a-IRES-Blast backbone (Addgene 85133). Spontaneous or experimental metastas...
BLAST analysis of the P. pastoris genome database using amino acid sequences of ScRtg proteins as the query indicated the existence of putative P. pastoris Rtg (PpRtg) homologues (Table 1). Because RTG signaling culminates in the activation of nuclear genes by the heterodimeric transcription ...