Here, the X-ray crystal structures of three NTF2-like proteins of unknown function are reported: those of ActVI-ORFA from Streptomyces coelicolor A3(2) and its homologs Caci_6494, a protein from an uncharacterized biosynthetic cluster in Catenulispora acidiphila , and Aln2 from Streptomyces ...
According to the BLASTP search result, SgcJ homologues feature a domain of unknown function (DUF4440) and belong to the NTF2-like superfamily, a large group of related proteins that share a common protein fold. The NTF2-like superfamily proteins are widely found in both prokaryotic and ...
localization signal (NLS). This protein also plays a role in the nuclear import of the small-GTPase Ran-GDP that is a central protein in various signal transduction pathways (e.g. mitotic spindle formation, nuclear envelope assembly, or responses to biotic stresses)53,54,55,56. In bacteria,...
G3BP1 (Ras-GTPase-activating protein SH3-domain-binding protein 1) is one of the key proteins crucial for SG assembly, often referred to as an SG nucleator [1,7]. Its interaction ranges from protein-protein to protein-RNA via specialized domains such as the nuclear transport factor 2-like...
Ras GTPase Activating Protein SH3 Domain Binding Protein (G3BP) is a potential anti-cancer drug target implicated in several cellular functions. We have used protein crystallography to solve crystal structures of the human G3BP1 NTF2-like domain both alone and in complex with an FxFG Nup repe...
We cloned the gene RHE_RS02845 encoding a predicted NTF2-like domain-containing protein in Rhizobium etli , and prepared U - 13 C/ 15 N-labeled protein samples for its three-dimensional NMR structural determination. Here, chemical shift assignments for both backbone and side-chain atoms are ...
In eukaryotes, messenger RNA (mRNA) is dependent on nuclear translocation mechanisms that export it from the nucleus into the cytoplasm, where protein translation occurs. NXF1-like members of the Nuclear eXport Factor (NXF) family orchestrate bulk nuclear export of mRNA, while functionally distinct...
Protein Structure, TertiaryTAP-p15 heterodimers have been implicated in the export of mRNAs through nuclear pore complexes (NPCs). We report a structural analysis of the interaction domains of TAP and p15 in a ternary complex with a Phe-Gly (FG) repeat of an NPC component. The...
The crystal structure of the NTF2-like domain of the Drosophila homolog of Ras GTPase SH3 Binding Protein (G3BP), Rasputin, was determined at 2.7 resolution. The overall structure is highly similar to nuclear transport factor 2: It is a homodimer comprised of a β-sheet and three α-...
Crystal structure of SgcJ, an NTF2-like superfamily protein involved in biosynthesis of the nine-membered enediyne antitumor antibiotic C-1027doi:10.1038/ja.2016.88Comparative analysis of the enediyne biosynthetic gene clusters revealed sets of conserved genes serving as outstanding candidates for the ...