Eight weeks’ post transplantation, the BM of recipient mice were comprehensively analyzed via flow cytometry (Fig.4A). Both BM and PB showed engraftment of human cells at a rate of 52.7 ± 35.1% and 18.2 ± 12.6%, respectively. 9.1 ± 6.5% of BM-resident human cells expr...
Additionally, as multiple integrations were determined to exist on mouse chromosome 2, hemizygous mice are estimated to have between 8 full copies (or 12–30 partial copies) (commissioned analysis by The Jackson Laboratory) which is essential to establishing such a robust model for SARS-CoV-2 ...
NSG mice (NOD.Cg-PrkdcscidIl2rgtm1Wjl/SzJ, 6-8 weeks old) from Jackson laboratory were irradiated (2.5 Gy) and then mice were subcutaneously injected with one million ffluc-zeo tumor cells. Tumor progression and tumor burden were monitored by Bioluminescent Imaging system using the Xenogen ...
Materials and Methods Mice. NSG mice were purchased from the Jackson Laboratory42 and bred in the Animal Facility at the Translational Research Institute (TRI) in Brisbane. Adult mice (2–4 months) were sublethally irradiated with 250 cGy using a Gamma Cell 40 Caesium source 24 hours ...
mice 1.3.产品编号 B-CM-002 1.4.基因型 Male: Prkdc (-/-), Il2rg (X-/Y); Female: Prkdc (-/-), Il2rg (X-/X-) 1.5.背景概述 B-NSG?(B-NDG?)小鼠(NOD-PrkdcscidIl2rgtm1/Bcgen)是百奥赛图公司自主开发的,NOD-scid遗传背景的IL2rg基因敲除小鼠,缺乏成熟的T、B和NK细胞,是目前国际公认的...
(one nucleotide in intron 5–6 and the remaining 26 nucleotides in the exon 6) Fig.1b and c. This allele was then maintained in NSG strain (Jax stock number 005557) to establish the Cmah−colony (Fig.1d). The NSG-cmah−/−mice are available from The Jackson Laboratory as NOD....
The cell-BME mixture (100 μl) was injected into the lactiferous duct of the fourth mammary glands of either six-week-old female athymic nude (Foxn1nu/nu, Harlan laboratories) mice or NSG mice (NOD.Cg-Prkdcscid Il2rgtm1Wjl/SzJ, Jackson laboratories) as described ...
Level of hCD45+ Cell Engraftment in the BM and Fold Increase in Engraftment Observed in NSG Mice Transplanted with CBSC and CB NK Cells.Michelle, EscobedoCousinNicola, JacksonRaquel, LazaBriviescaLinda, ArizaMcNaughtonMartha, LuevanoSophie, Der...
Mice used in this study were 18–21-week-old NOD, Cg-PrkdcscidIl2rgtm1Wjl/SzJ (NSG) males, 22-week-old NSG females, and 25-week-old NSG females engrafted with human CD34+ hematopoietic stem cells (HIS mice). All animals were purchased from the Jackson Lab (USA) and, upon arrival, ...
The NSG-SGM3 mice (NOD.Cg-Prkdcscid Il2rgtm1Wjl Tg(CMV-IL3,CSF2,KITLG) 1Eav/MloySzJ, JAX stock #013062) were obtained from The Jackson Laboratory (Bar Harbor, ME, USA). This strain was originally described in Wunderlich et al., 2010 [66] and Billerbeck et al., 2011 [67]. Bo...