flavefaciens FD-1 genomic DNA by PCR and using the primers shown in Table S3. The recombinant dockerin encoded by the pHTP2 derivatives contained an N-terminal thioredoxin A and an internal hexa-histidine tag for increased protein stability and solubility. Sequences of all plasmids produced ...
The NS3 region was obtained via PCR using pBRTHCV (kind donation from Dr. Giuseppe Ciaramella, Pfizer) as template and the following two primers: forward, 5′-cgcggtacc atggcgcccatcacggcgtac-3′, and reverse, 5′-gggaattc ttaatgatggtggtgatggtgcgtgacgac-3′. Underlined nucleotides ...