Calcium-coordination geometry at the N-terminal and C-terminal F-hand motifs ofR. flavefaciensDocScaA. Panels A and B show a representation ofRfDocScaA N- and C-terminal Ca2+-coordination regions, respectively. In both panels the amino-acid residues involved in the metal coordination are depict...
The NS3 region was obtained via PCR using pBRTHCV (kind donation from Dr. Giuseppe Ciaramella, Pfizer) as template and the following two primers: forward, 5′-cgcggtacc atggcgcccatcacggcgtac-3′, and reverse, 5′-gggaattc ttaatgatggtggtgatggtgcgtgacgac-3′. Underlined nucleotides ...