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Intact mRNA was first isolated from total RNA samples using an mRNA isolation kit per the manufacturer’s protocol (Promega). MeRIP was performed as previously described [24]. The isolated mRNA was chemically fragmented into 200 nucleotide-long fragments by incubating at 94 °C for 5 min. Then...
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24 hours post transfection, 200 μ l of supernatant was transferred to Scientific Reports | 5:11346 | DOI: 10.1038/srep11346 13 www.nature.com/scientificreports/ MDCK-II cells (6 well format). Recombinant viruses were plaque-purified upon the observation of cyto- pathic effects. ...
We have explored their utility on a study area of approximately 8400 km2 to the north of Quebec City, to extract arborescent composition of forest cover from Landsat TM-5 satellite images, map data and ground survey plots. (Abstract shortened by UMI.)...
Cells were fixed in 200 mM Na-cacodylate buffer containing 180 mM glucose, 6% formaldehyde and 4% glutaraldegyde and 30, 50, 70 or 90% absolute ethanol on 5 nm thick gold-palladium grids. Results and discussion General features The genome of D. phosphitoxidans contains one circular ...
For example, If you are building docker images with heroku yml, you need to change the stack to container. You can use the stack option to change stack for your app. .github/workflows/main.yml name: Deploy on: push: branches: - master # Changing the branch here would also work jobs:...
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**Versatile Shooting with 4K Video and 8x Optical Zoom** This camera is not just about still images; it also excels in video recording. With 4K HD video capabilities, you can capture life's moments in stunning detail, perfect for creating professional-quality videos. The 8x optical zoom ...
echo "Push images" docker push $IMG fi echo "Generating yaml" sed -e 's@image: .*@image: '"${IMG}"'@' config/manager/network.yaml > $DEST kubectl apply -f $DEST kubectl apply -f config/rbac/rbac_role_binding_network.yaml170...