(control gene for normalization of transfection efficiency and cell growth; Promega, Fitchburg, Wisconsin, USA) and one pFA-CMV-hNR-LBD clone coding for the hinge region and ligand binding domain (LBD) of a human NR of interest, or with pECE-SV40-Gal4-VP1643 (gift from Lea Sistonen; ...
the cells were incubated with the test compounds by changing the medium to Opti-MEM supplemented with penicillin (100 U/mL) and streptomycin (100 μg/mL) additionally containing 0.1% DMSO and the respective test compound or 0.1% DMSO alone as untreated control. After overnight (14–6 ...
Department of Biochemistry, University of Wisconsin-Madison, 433 Babcock Drive, Madison, 53706, Wisconsin, USA Douglas B. Weibel Department of Chemistry and Chemical Biology, Harvard University, 12 Oxford Street, Cambridge, 02138, Massachusetts, USA Douglas B. Weibel, Willow R. DiLuzio & George ...
(control gene for normalization of trans- fection efficiency and cell growth; Promega, Fitchburg, Wisconsin, USA) and one pFA-CMV-hNR-LBD clone coding for the hinge region and ligand binding domain (LBD) of a human NR of interest, or with pECE-SV40-Gal4-VP1643 (gift from Lea Sistonen...
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Genome editing of a human episomal iPSC line (Thermofisher #A18945) was performed by Cellular Dynamics International (Madison, Wisconsin). Nucleases were designed to target the genome close to the desired modification areas (exon 4 of NM_005123 forNR1H4knockout, or codon R436 forNR1H4R436H...
One answer to this question has recently been provided by a team at the University of Wisconsin. Reporting in Science, Jon Thorson and colleagues have elucidated the mechanism by which actinomycetes are self-resistant to the antibiotic calicheamicin γ1 — an enediyne that acts as a DNA-cleaving...
One answer to this question has recently been provided by a team at the University of Wisconsin. Reporting in Science, Jon Thorson and colleagues have elucidated the mechanism by which actinomycetes are self-resistant to the antibiotic calicheamicin γ1 — an enediyne that acts as a DNA-cleaving...
Lipton, M. S. et al. AMT tag approach to proteomic characterization ofDeinococcus radioduransandShewanella oneidensis.Methods Biochem. Anal.49, 113–134 (2006). CASPubMedGoogle Scholar Elias, D. A. et al. Global detection and characterization of hypothetical proteins inShewanella oneidensisMR-1 us...
One answer to this question has recently been provided by a team at the University of Wisconsin. Reporting in Science, Jon Thorson and colleagues have elucidated the mechanism by which actinomycetes are self-resistant to the antibiotic calicheamicin γ1 — an enediyne that acts as a DNA-cleaving...