To introduce plasmids constructed as described above, the host CHO cells are first seeded at a density of 5×105in 10% FBS-supplemented MEM medium. After growth for 24 hours, fresh medium are added to the plates and three hours later, the cells are transfected using the calcium phosphate-DN...
Assignee: NPS Pharmaceuticals, Inc. (Salt Lake City, UT) Kirin Beer Kabushiki (Tokyo, JP) Primary Class: 514/654 Other Classes: 514/655, 564/341, 564/353, 564/354 International Classes: A61K31/165; A61K31/4035; A61P5/20; C07C211/30; C07C217/20; C07C217/58; C07C237/06; ...
As of August 10th, the pilots of the ten to 17 military, CAP and civilian aircraft committed to the search each day since it began had flown 148 sorties covering 2,450 square miles and had logged almost 500 person/hours in the effort. Separate ELT signals were identified in the search ar...
D-Xylose was used to test intestinal carbohydrate absorption. After an overnight fast, patients drank a test solution of 25 g D-Xylose in 200-mL distilled water over a 2-3 minute period. A blood sample was taken at two hours and urine collected for 5-hours following ingestion. Morphologica...
ALLEN C TURNER (SALT LAKE CITY, UT, US) Claims: What is claimed is: 1. A process for preparing an R enantiomer of a compound having a formula (I): ##STR10## wherein Me is methyl, Ar is 3-methoxyphenyl, 3-chlorophenyl, or 1-naphthyl, and X is independently selected from the gro...
The sample was digested for 3 hours in 37 C water block heater, heat-inactivated at 65° C. for 20 min and purified using GeneCleanII kit from BIO 101. Aliquots of the PCR reactions were cloned into the above pBluescript plasmid vector using T4 DNA ligase kit (New England Biolabs) and...