Some third parties are outside of the European Economic Area, with varying standards of data protection. See our privacy policy for more information on the use of your personal data. Manage preferences for further information and to change your choices. Accept all cookies ...
coli BL-21 (DE3) cells (SI10). Subsequently co-crystals of PPARγ were grown using 6 mg ⋅ ml-1 protein in buffer: 20 mM Tris-HCl pH 8.0, 1 mM TCEP, 0.5 mM EDTA and 1 mM design 1 mixed with equal amounts of reservoir: 0.1 M Tris-HCl pH 7.5 and 1.6 M ...
Some third parties are outside of the European Economic Area, with varying standards of data protection. See our privacy policy for more information on the use of your personal data. Manage preferences for further information and to change your choices. Accept all cookies ...
Chromatin is dynamically reorganized when DNA replication forks are challenged. However, the process of epigenetic reorganization and its implication for fork stability is poorly understood. Here we discover a checkpoint-regulated cascade of chromatin si
In an increasingly competitive environment, we must be able to act quickly and deci- sively. This requires us to simplify the way we operate, and empower leaders at all levels of the organisation to make the decisions that are necessary in order for their area of the business to achieve ...
Using a custom-made stainless-steel punch (square base with 96 bars with diameter of 0.5 cm), pieces weighing an average of 45.3 mg were extracted and placed into individual wells of a 96-well plate. To ensure uniform positioning at the bottom of the wells, the plates were centrifuged (...
Use freshly made lithium acetate working solution for transformations. PEG working solution (2.5 mL) ReagentFinal concentrationAmount 1 M lithium acetate 100 mM 0.25 mL 50% PEG 40% 2 mL 10X TE 1X 0.25 mL Add 250 μL of autoclaved 1 M lithium acetate and 250 μL of autoclaved 10X TE ...
We applied ScanIndel on both 300-bp and 150-bp read data sets and compared it with other widely used NGS tools to test their performance on the 300-bp read data set (see Methods for details). The results achieved with each software tool are summarized in Table 1. Only ScanIndel detected...
Some third parties are outside of the European Economic Area, with varying standards of data protection. See our privacy policy for more information on the use of your personal data. Manage preferences for further information and to change your choices. Accept all cookies ...
As expected, the evidence code distribution shows an over-representation of electronic annotations (IEA), although other non-automatic codes, such as Inferred from Direct Assay (IDA) and inferred by mutant phenotype (IMP), were also well represented (see bar-plot in Additional file 6). A. ...