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(YNB) medium with following composition was used to select auxotrophic Ura− and Leu− transformants and to prepare the seed cultures: 6.7 g/L yeast nitrogen base (without amino acids w/ammonium sulfate) (Becton, Dickinson and Company, Sparks, MD, USA), 20 g/L glucose, and 1.92 g/L...
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In the latter, for example, the addition of small crystals of sodium chloride to a saturated solution of this salt can seed the formation of more crystals. This may be considered as reproduction, but this system cannot undergo Darwinian evolution as there is no possible way that the structure...
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