could thermodynamically outcompete the D5 interfaces. To test this, we used a globular protein version of the effector, 3hb21, which binds the hinge tenfold more strongly11. We found that, as anticipated and in contrast to the peptide effector, the stabilized protein version of the effector i...
The HB1.9549.2 protein (cyan) was grafted into a parental six-helix bundle (sCage, yellow) at different positions along the latch helix (magenta), including three consecutive glycine residues (green). The black arrows indicate the additionally introduced single V255S (1S) or double V255S/I...
sheets of the designs and NMR structures. (bottom) Hydrogen-bond (HB) formation probability for each hydrogen bond (The numbering in the bar graph corresponds to that shown in the β-sheet schematics). Red arrows show the incomplete hydrogen bonds, in which donor is provided by the first res...