NuPAGE® Tris-Acetate SDS Running Buffer for NuPAGE® Novex Tris- Acetate Gels NuPAGE® Transfer Buffer for blotting of NuPAGE® Novex Pre-Cast Gels NuPAGE® Bis-Tris Discontinuous Buffer System The NuPAGE® Bis-T...
4) Remember that if your target protein has a low MW (≤10 kDa), it may transfer more quickly than expected. 5) If you are using PVDF membranes, make sure to pre-soak the membrane in methanol first before soaking it in transfer buffer. Note that methanol in transfer buffer increases ...
native proteins from NativePAGE™ gels to PVDF membranes after BN PAGE separation. This transfer protocol uses a neutral pH transfer buffer without SDS and a transfer time of 7 minutes with the iBlot™ Dry-Blotting System to achieve fast ...
This can happen if Tris-Glycine gels are run using NuPAGE Running buffer containing Antioxidant. Please make sure that the correct Tris-Glycine Running buffer is used with Tris-Glycine gels. Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotti...
On the other hand, NuPAGE LDS sample buffer (pH 8.5) drops to pH 8.1 when heated to 70 degrees C, avoiding this cleavage. *Faster run times: 35-50 min for NuPAGE Bis-Tris gels and 1 hour for NuPAGE Tris-Acetate gels vs 90 min for Tris-Glycine gels Find additional tips, ...
On the other hand, NuPAGE LDS sample buffer (pH 8.5) drops to pH 8.1 when heated to 70 degrees C, avoiding this cleavage. *Faster run times: 35-50 min for NuPAGE Bis-Tris gels and 1 hour for NuPAGE Tris-Acetate gels vs 90 min for Tris-Glycine gels Find additional tips, ...
*NuPAGE Tris-Acetate gels: For Native electrophoresis, use Invitrogen Tris-Glycine Native Running Buffer. For Denaturing electrophoresis, use NuPAGE Tris-Acetate SDS Running Buffer *NuPAGE Bis-Tris gels: For Denaturing electrophoresis, use NuPAGE MOPS-SDS Running Buffer or NuPAGE MES-SDS Running ...
We recommend checking the transfer buffer and its reagent components and re-diluting or remaking the buffer. Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center. NuPAGE gels have the following advantages over Tris-Glycine gels:...
Mini Gel Tank 小型胶电泳槽兼容所有 Invitrogen Novex、NuPAGE 和 Bolt 微型凝胶。每个微型凝胶槽每次可最多容纳两块凝胶。独特的凝胶罐设计可实现便利的凝胶并行加载,加强使用期间的观察。运行时间可能因所使用的缓冲条件和电源而异。 Novex Tricine 迎新套装包含:• 小型凝胶槽 (A25977)• Novex Tricine 小型...
Introducing iBlot 3 Western Blot Transfer System Featuring higher throughput and built-in cooling for consistent protein transfer Learn more › Learn more About the Novex IEF gels › Related Western Workflow Products Novex IEF Sample Buffer pH 3-10 (2X) › Novex IEF Sample Buffer pH 3-7 ...