novella sequence had a minimum length of 233 amino acids and a minimum similarity value of 39% over this length. The SoxX sequence alignments varied in length between 77 and 211 residues. Over a 77-amino acid alignment of the R. sulfidophilum and S. novella sequences, a similarity of 49...
An alternative to this subcloning step is the transfer of the RP4 mob locus and tet genes into the pDorEX construct as set out in [21]. An interesting observation in relation to expression control is that, while the chromosomal copy of the dor promoter is tightly controlled and DMSO ...