Bacterial expression and tagging vectors Try SnapGene for Free Download SnapGene Viewer Explore Over 2.7k Plasmids:More Plasmid Sets Individual Sequences & Maps pACYCDuet-1 pET-24a(+) pET-3a pET-51b(+) Ek_LIC (linearized) pCDF-1b pET-24b(+) ...
ChoosingavectorandcloningstrategyPart2:SelectionofhostandoptimizationofexpressionPart3:MaximizingtargetproteinrecoveryProteinExpressionBasedonBacterialVectorHostGrowthconditionsThingsyoushouldconsiderwhenchoosingvectorsLevelofcontrolandexpressionFusionTagsProteaserecognitionsiteAntibioticselectionCopynumberWhatdoesatypicalvectorcontain...
Things you should consider when choosing vectors Level of control and expression Fusion Tags Protease recognition site Antibiotic selection Copy number Page 4 Editor: Presentation name here 2 What does a typical vector contain? xx/xx/xxxx Page 5 You have more than you want… Editor: ...
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