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Hi all! I'm not a chemist but I'm writing a short story that uses some chemistry to resolve the plot. If anyone is willing to critique my ideas for how I'm going to get my character out of the jam they're in (or provide any even better idea from the materials the character has...
Briefly, cells were rinsed promptly in PBS buffer, fixed with formalin for 10 min and rinsed in distilled water. TRAcP was cytochemically demonstrated by a simultaneous coupling reaction using Naphtol AS-BI-phosphate as substrate and Fast violet B as the diazonium salt. Cells were then ...
For differentiation analyses, cells were harvested from the well of a 6-well plate, and 1mL of pre-warmed detachment buffer (1xPBS, 0.144 g glucose, 4.8mL EDTA (0.5 M) to an 800mL final volume) was added and incubated for two minutes before addition to the harvested cells. All cells...
and an final elongation at 72 °C for 10 min. We perform PCR reactions into a 25-ml reaction volume (1X PCR buffer, 2,2 mM MgCl2, 0.5 mM of each dNTPs, 0.55 µM of each primer, 0.02 U ofTaqpolymerase (GoTaq Promega), and 20 ng genomic DNA). The PCR products were purified ...
A recording and reproducing apparatus and method which prevents or managing a data loss caused by each of seek operations in recording/reproducing a time-series digital data set, to/from a disk recording media via a buffer. Beforerecordi... R Yasukohchi,A Uesugi,M Sato,... 被引量: 19发...
H-7 is a well-documented inhibitor of several protein kinases, including protein kinase C; however, it does not rapidly enter adipocytes protected with the intact plasma membrane. The cells were suspended in Buffer X [4.74 mM NaCl, 118.0 mM KCl, 0.38 mM CaCl2, 1.00 mM EGTA, 1.19 mM Mg2...
The results show that, indeed, for metal containing species in this size regime, reaction with molecular nitrogen has a dramatic effect on measured drift times and must not be ignored when comparing and interpreting TWIMS arrival time distributions. Density-functional theory (DFT) calculations are ...
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At pH 6.8 (buffer), the half-life of the magnesium salt is about 19 hours at 25°C and about 8 hours at 37°C. 12 CLINICAL PHARMACOLOGY 12.1 Mechanism of Action Naproxen and esomeprazole magnesium delayed-release tablets consists of an immediate-release esomeprazole magnesium layer and an ...