图1 Nos1Cre/+; Rosa26tdTomato/+小鼠脑部tdTomato的表达情况。 Nos1Cre/+; Rosa26tdTomato/+双转基因鼠,可在小鼠嗅球、皮层和海马中检测到tdTomato的表达。 图2 Nos1Cre/+; Rosa26tdTomato/+小鼠大肠中tdTomato的表达情况。 Nos1Cre/+; Rosa26tdTomato/+双转基因鼠,可在小鼠大肠肌肉层和粘膜层的细胞...
金致和 GENEANDPEACE 小鼠品系说明书 B6.129-Nos1tm1(cre) 品系编号:GAP1047 品系简称:Nos1cre 品系特点: Nos1cre 敲入小鼠有助于产生条件突变以研究新陈代谢和能量稳态. 遗传学信息: 遗传背景:C57BL/6 品系类型:靶向突变 相关基因:Nos1 饲养信息: 配繁策略: Homozygote x Homozygote 配繁特性: 当维持种群...
接着,课题组在nNOS-Cre小鼠的前囟后1.4cm的BLA脑区注射了AAV-CaMKⅡ-DO-hM4Di-eGFP,等病毒表达21天后,分别在dBNST、vHPC套管给予CNO,特异性抑制BLA-dBNST或者BLA-vHPC环路。结果发现:抑制BLA中Nos1-神经元到dBNST环路后,小鼠的旷场、高架十字迷宫、悬尾、强迫游泳检测结果与对照组无明显差异;但是,当特异性抑...
Nos1-IRES-Cre 品系名:C57BL/6Smoc-Nos1em1(IRES-iCre)Smoc 品系状态:精子冻存 | 目录号:NM-KI-200113 Nanos1-Flox 品系名:C57BL/6Smoc-Nanos1em1(flox)Smoc 品系状态:在研 | 目录号:待定 Nos1ap-Flox 品系名:C57BL/6Smoc-Nos1apem1(flox)Smoc 品系状态:在研 | 目录号:待定你...
Results: To determine the significance of the macula densa NOS1, we measured glomerular filtration rate (GFR) and mean arterial pressure (MAP) with telemetry in macula densa specific NOS1 knockout (KO) mice (NKCC2cre; NOS1flox/flox) and WT (NOS1flox/flox) mice. In the WT ...
To determine the role of Nos1(PVH) neurons in energy balance, we used Cre-dependent viral vectors to both map their efferent projections and test their functional output in mice. Here we show that Nos1(PVH) neurons project to hindbrain and spinal cord regions important for food intake and ...
Pyramidal expression of mouse Nos1 mRNA was confirmed by quantitative RT-PCR of fluorescently sorted pyramidal neurons fate mapped in mice doubly transgenic for Emx1-Cre and a CRE-responsive Gfp (CAG-Cat-Gfp) (Figure 4C). Therefore, whereas NOS1 mRNA is expressed in pyramidal neurons of both...
随后,课题组在nNOS-Cre小鼠的前囟后1.9cm的BLA脑区注射了AAV-CaMKⅡ-DIO-hM3Dq-eGFP,等病毒表达21天后,腹腔注射CNO,电生理检测,确实能引起BLA脑区Nos1+神经元的放电频率增加。兴奋BLA脑区Nos1+神经元,小鼠的运动能力无变化,但是,高架十字迷宫开臂的滞留时间增多,旷场中央区的总距离增加,悬尾的不动时间减少,强...
将CreERT2-SV40polyA插入到小鼠Nos1基因起始密码子处。 应用领域:Nos1,神经源性一氧化氮合酶,是负责神经元产生信号分子一氧化氮 (NO) 的关键酶。Nos1-CreERT2小鼠与含有 loxP 位点侧翼序列的株系杂交时,他莫昔芬诱导后,Cre 介导的重组会导致后代中该序列的组织特异性缺失。 *使用本品系发表的文献需注明:...