that trapped air bubbles between the layers are removed (for example, by rolling a glass pipette across the surface). Place the transfer sandwich in the correct orientation into the tank electroblotter and fill up with 1× TBE. Move the chamber to 4°C and start transfer 50V for 1h at ...
Tank The Prank The Spy eschews pranks for mundane topics such as AI and Transhumanism When The Months Go Marching On Implementing fully electric vehicles, the Spy's home network project, and the need to kneed the knee. To Rue or Not to Rue ...
Once the electrophoresis process is complete, the RNA gel is removed from the tank and rinsed with water. An oblong sponge that is slightly larger than the gel is placed on a glass dish, and the dish is filled with SSC to a point so as to leave the soaked sponge about half-submerged ...
Location of the (a) western and northern Antarctic Peninsula and the (b) Gerlache Strait, with a simplified surface circulation pattern (red arrows) that is strongly influenced by the Bellingshausen Sea. The surface circulation in (b) was based on Savidge and Amft98. The dashed red arrows ...
Oxygen depletion constitutes a major threat to lake ecosystems and the services they provide. Most of the world’s lakes are located >45° N, where accelerated climate warming and elevated carbon loads might severely increase the risk of hypoxia, b
BCE phase of construction, running west to east, with a downward slope toward a circular tank, was closed probably before the 2nd c. CE, with the imposition of the tannery [62] (p. 116) (Figure 23). However, it confirms the downward slope we registered in the floor and street ...
Smart Cities and Urban ManagementG2: Phase Change Materials for Energy StorageH: Geo-EnergyH1: Petroleum EngineeringH2: GeothermalH3: FossilI: Energy Fundamentals and ConversionI1: FuelI2: Energy and Combustion ScienceI3: Energy ChemistryJ: Thermal ManagementJ1: Heat and Mass TransferJ2: Thermodynami...
Environmental protection is becoming a primary objective when choosing processes and technologies for treating oily waters resulting from hydrocarbon production. The focus of treating oily waters is to eliminate contaminants from effluents before dischar