They aren’t that’s my issue. When I turn off my GPU fans, and 100% my case / intake fans (5) the temperature stays the same and doesn’t drop noticeably. I don’t see the impact. On my GPU the fin stack is completely covered in a plastic shroud. That's a bad designed GPU ...
Cells were fixed in ice-cold fixative (4% paraformaldehyde and 0.5% glutaraldehyde in PBS) and incubated for 18 min at room temperature in the dark, followed by permeabilization with 0.2% Triton X-100 in PBS for 4 min. Cells were then cultured with primary antibodies in IF buffer (TBS ...