evidentfromanumberoffacts:fl1theself-limitingnatureoftheinfection(RobertsThomsonela1.,l976),(21theappearanceofG./ambliaspecificantibodiesintheseraofinfectedpatients(Radulescueta1.,1976;Ridleyela1.。l976andSmithela1.,l981),(3)anincreasedsusceptibilityofinfectioninhypo.)1.globulinemicpersons(Amentela1.,...
Cells were plated in 24-well plates (ibidi USA Inc., WI, USA), washed twice with phosphate-buffered saline (PBS), fixed with 1% paraformaldehyde in PBS for 10 min, permeabilized with 0.5% Triton X-100 in PBS, and washed twice with PBS. A 15 min incubation with Blocking One (Naca...
environment. On-chip drug screening and cell sorting systems based on electrical sensing and mechanical stiffness assessment have also been developed to analyze cell cultures at a single-cell level36,37. However, a majority of these technologies have been limited to the laboratory scale owing to ch...
The cells were then washed with phos- phate-buffered saline (PBS) then plated in 25 cm2 culture flasks at passage 1, at a ratio of 1:4. The cells were main- tained at 37 °C within a humidified atmosphere contain- ing 5% C O2. The culture medium was refreshed every ...