Body temperature 37.5°C Basal metabolism rate (400 gm rat) 35kcal/24hr Chromosome number (diploid) 42 Puberty 50 ± 10 days Gestation 21–23 days Litter size 8–14 Birth weight 5–6gm Eyes open 10–12 days Weaning 21 days Food consumption/24 hr 5 gm/100gm body weight Water consumptio...
Prolonged Exposure to Heat The scrotum houses the testes and regulates the temperature for optimal sperm production. It is designed to keep the testes slightly cooler than the body’s core temperature, which is why they are external organs. This temperature difference is essential for producing he...
Frozen tissues were embedded in optimal cutting temperature (OCT) compound and cryo-sectioned at 25 μm on PEN-membrane slides and fixed in 70% ethanol for 5 min. Fixed tissue sections were rinsed twice with 1X PBS, stained with haematoxylin and eosin (H&E), and photo-recorded using ...
Obesity is a prominent risk factor for male infertility, and a high-fat diet is an important cause of obesity. Therefore, diet control can reduce body weight and regulate blood glucose and lipids, but it remains unclear whether it can improve male fertil
The incubation at 37掳C is more favorable for aPL binding than ambient temperature. Normal human serine (IgG-NHS) did not elicit any distinct change of NBD-PE fluorescence, which indicates it does not interact with the lipid.doi:10.1016/S0005-2736(98)00028-5Daxin Tang a...
Walmart published its 6-20-100 guidance: stand six feet away to maintain a safe physical distance, take 20 seconds for good hand washing, consider a body temperature of 100 degrees Fahrenheit the signal to stay home from public activity. Slack CEO Stewart Butterfield focused on pers...
Minor changes may be due to drinking too much or too little fluid that day. Stools can also change based on your diet, physical activity, temperature, and other factors. Checking too often can make you hypersensitive to things that may or may not matter, in some cases placing you in a ...
Following 6 × 10 minute washes in 0.1M PBS, Alexa Fluor 680 donkey anti-Rabbit Ig (H + L) (Abcam ab186692, 1:10000) was added and membrane incubated for 90 minutes at room temperature. Blots were washed 6 × 10 minutes in 0.1 M PBS and ...
above. The localisation of the biotin tracer was visualised by incubation with Streptavidin Alexa Fluor™ 546 conjugate (S11225, Thermo Scientific, UK), diluted 1:200 in PBS, for 1 hr at room temperature. Tissue sections were imaged using an LSM 780 confocal microscope (Carl Zeiss, UK)....
Cells were then cultured with primary antibodies in IF buffer (TBS plus human serum cocktail) overnight at 4 °C in a humidifier chamber. Next day, cells were washed three times in PBS, and cells were incubated with secondary antibodies in blocking buffer for 1 h at room temperature. After...