Hemoglobinized cells comprised less than 2% of nucleated cells in the marrow aspirate. Patients with Diamond-Blackfan anemia had marrow erythroid hypoplasia and either a high mean corpuscular volume or fetal hemoglobin level. All patients met standard diagnostic criteria.14 Eight patients in our study...
Primers and probes were designed based on NCBI transcript sequence NM_000518.4 (HBB), mRNA sequence information from Vadolas et al. [4] (HBBIVSI−110)and NCBI genomic DNA Reference Sequence NG_000007.3 (HBBlocus), and were tested for self-annealing and hairpin structures using Vector NTI ...