Hb type was characterized in 125 samples: 45 (36%) individ- uals had carried a fraction of fetal H b with the IEF pattern of +RBC 10"/2 y 1.3 , 20 RDW 4 Y r = 0.507 ,, 25 30 x- weeks H b F Sardinia (775 Thr-136 Ala) (7); samples with a different chain mutation have...
MRI was performed at gestational weeks 20.1–40.6 in a 1.5T system using 10 b -values (0–1000s/mm2). Regions of interest were drawn covering the entire placenta in five transverse slices. Diffusion coefficient (D), pseudodiffusion coefficient (D*) and perfusion fraction (f) were estimated ...
Breast tissues (plastic surgery or breast cancer) were kept at 4°C in complete medium, (DMEM medium with 10% fetal bovine serum (FBS)) and processed within 1-2 hours. Tissues were minced, transferred to collagenase digestion medium (Stem Cell Technologies (SCT), Vancouver, Canada) and ...
Human fetal prostate (15–18 weeks) was also included in the analysis to establish mRNA expression levels in devel- opment. The CAF/NPFs were functionally tested using previously described methods; CAFs produced tumours when recombined with an epithelial cell line, whereas NPF samples did not (...
The patient is a girl, now 11 years old, born three weeks preterm by section due to transverse lie, birth weight 2720 g. There were no feeding difficulties in infancy or later. Motor and language development was delayed: she walked without support at age 2½ years, and at age 3 years...
Continuous cell lines were grown in RPMI-1640 or Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10% fetal calf serum (FCS), at 37°C in an atmosphere of 5% CO2in air. PAP staining. Cytocentrifuge preparations were air-dried over- night and fixed for 1 minute in acetone ...
All cell lines used in this article were obtained from our own laboratory of the Department of Cell Biology at Soochow University. Cells were main- tained in MEM medium (Gibco-BRL, Shanghai, Chi- na) supplemented with 10% Fetal Bovine Serum (FBS) (Sino-American Biotechnology Co, Shanghai,...
The two liquid phases were collected separately and dried while a solid protein precipitate remained at the bottom on the extraction tube (Fig. 1). Figure 1 Workflow of the Serial-omics experiment. 10 mg of breast tumor tissue and 10 mg of normal mammary gland were harvested from the ...
(100, 95, 70%) and deionized H2O. Antigen retrieval was performed in 10 mM Tris, 1 mM EDTA, 0.05% Tween 20, pH 9 at 121 °C for 4 min. Subsequently, tissue sections were blocked in 1% bovine serum albumin, 2% fetal bovine serum in PBS for 5 min, and incubated with ...
that calcium-sensing receptors are required for the homing of fetal liver HSCs to the endosteum,32indirectly suggesting a role for [Ca2+]ein stem cell retention. However, it was also recently demonstrated that [Ca2+]ethat is at least 10-fold lower than the serum calcium concentration plays a...