The total number of protein-coding genes in the human genome is not significantly higher than those in much simpler eukaryotes, despite a general increase in genome size proportionate to the organismal complexit
The genome is pervasively transcribed but most transcripts do not code for proteins, constituting non-protein-coding RNAs. Despite increasing numbers of functional reports of individual long non-coding RNAs (lncRNAs), assessing the extent of functionality among the non-coding transcriptional output of ...
Genome and transcriptome projects have revealed, however, a significant number, rivaling the protein-coding transcripts, of non-protein-coding RNAs not related to these previously characterized transcript classes. Non-protein-coding RNA research has primarily focused on microRNAs, a small subclass of ...
CDS, coding sequence; HEK, human embryonic kidney; NTR, noncoding transcript. Virotrap Data of a Selected NTR Protein To further investigate the functionality of NTR proteins, we selected one NTR protein to study its interactome by Virotrap. An Nt-peptide pointing to an Nt-proteoform (missing...
In these years a huge number of human transcripts has been found that do not code for proteins, named non-protein coding RNAs. In most cases, small (miRNAs, snoRNAs) and long RNAs (antisense RNA, dsRNA, and long RNA species) have many roles, functioning
Targeted protein degradation has become a notable drug development strategy, but its application has been limited by the dependence on protein-based chimeras with restricted genetic manipulation capabilities. The use of long non-coding RNAs (lncRNAs) has
Long non-coding RNA has been involved in cancer progression, and high HOX transcript antisense intergenic RNA (HOTAIR) is thought to be a poor prognostic i... Qiwen,Deng,Huiling,... - 《Plos One》 被引量: 46发表: 2014年 Clinical relevance of long non-coding RNA in acute myeloid leukemi...
Neurodegeneration is clearly not exclusively caused by imbalances in protein coding genes—it can also arise from dysregulation of ncRNAs. Over the past two decades, we have begun to understand that ncRNAs are not just “transcriptional noise” and have started to define their role in the CNS and...
RNA-protein interactions can occur either in the cytoplasm or nucleus, and RIP can be applied to either total or fractionated (nuclear or cytoplasmic) transcript pools. Active Motif’sRNA ChIP-IT® kit, though, is specifically designed to detect RNA interactions with nuclear chromatin,...
which contains the CREB-binding site in HCC cells.99It has been suggested that lncRNAs located near protein-coding genes may have a role in regulating the expression of their neighboring protein-coding genes. Indeed, Duet al.showed that overexpressed HULC downregulates a downstream gene, p18, whic...