Kupffer cellsHepatic stellate cellsHepatocytes comprise the majority of liver mass and cell number. However, in order to understand liver biology, the non-parenchymal cells (NPCs) must be considered. Herein, a relatively rapid and efficient method for isolating liver NPCs from a mouse is described...
human hepatic non‐parenchymal cellsh‐NPCshuman liverHuman induced pluripotent stem cells (h﹊PSCs) represent a potentially unlimited source for the generation of human hepatocyte﹍ike cells (h﹊HLCs) for the establishment of platforms to study drug﹊nduced hepatotoxicity, liver disease modeling, ...
DNA strand breaks (nicks) in non-parenchymal cells (NPCs) in CCl 4 -induced acute or chronic liver injury in rats were detected using an in situ nick translation method; their dynamic changes were analysed in relation to the proliferation pattern of hepatocytes and NPCs, as revealed by ...
Hepatic stellate cellsMyofibroblastsFibulin-2 and cytoglobin/stellate cell activation-associated protein (Cygb/STAP) are considered to be markers of hepatic myofibroblasts (MFs) and stellate cells (HSCs), respectively. The aim of the present study was to characterize the nonparenchymal cells (NPCs) ...
by injection of pig serum.Liver fibrogenesis was detected by Masson's trichrome staining.Rat non-parenchymal cells(NPCs)were enriched 4-fold by Percoll density gradient centrifugation.Protein extracts from NPCs were prepared at 4 and 8 weeks,separated by two-dimensional electrophoresis,and then ...
After depletion of YAP in HCs and BECs, YAP expression was induced in non-parenchymal cells (NPCs) in a cholestasis-independent manner. YAP positivity was detected in subgroups of Kupffer cells (KCs) and endothelial cells (ECs). The secretion of pro-inflammatory chemokines...
Hepatocytes account for about 60% of the total cell population in human liver, with non-parenchymal cells (NPCs) encompassing the other 40%. Among NPCs, liver sinusoidal endothelial cells (LSECs) contribute ∼40%, Kupffer cells (KCs) ∼30%, hepatic stellate cells (HSCs) 10%–25%, and...
Objective The development of alcoholic liver fibrosis( ALF) is a complex process involving both parenchymal and nonparenchymal cells. The knowledge about the proteome of liver nonparenchymal cells( NPCs) in response to ethanol is limited. This paper aims to investigate the regulatory effect of ...
(DILI)may provide new strategies for its prevention and therapy.However,little is known about the underlying mechanisms of this phenomenon.We used single-cell RNA sequencing to characterize the dynamics and functions of hepatic non-parenchymal cells(NPCs)in autoprotection against DILI,using ...
The scope of this study was to investigate whether cocultivation of hepatocytes with NPCs would augment the functions of a BAL (HN-BAL) compared with a BAL equipped with only hepatocytes (H-BAL). In addition, NPCs were similarly cultivated alone. The cells were cultivated for 8 days in ...