Here, we studied in an MHC-matched, minor antigen mismatched HCT model (C3H.SW脿C57BL/6 (B6)) the effects of lethal irradiation and allo-HCT of HSC (cKIT+Sca1+Lin-) alone or in combination with T cells (TC) on the hematopoietic compartment (HC) and non-hematopoietic compartment (...
We assessed whether a hierarchical relationship might exist betweenVwf-tdTomato−multi-HSCs andVwf-tdTomato+P-HSCs (Fig.2a). The LSKCD150+CD48−phenotypic HSC compartment replenished in the BM of recipients of a single transplantedVwf-tdTomato−multi-HSC remained exclusively or predominantlyVwf...
Furthermore, our data show that PI3Kγ action on diet-induced obesity depends on PI3Kγ activity within a nonhematopoietic compartment, where it promotes energetic efficiency for fat mass gain. We also show that metabolic modulation by PI3Kγ depends on its lipid kinase activity and might involve...
On the other hand, loss of cyclin E1 provided HSCs with a competitive advantage in bone marrow serial transplantation assays, suggesting that a partial impairment of cell cycle entry may exert a protective role by preventing premature depletion of the HSC compartment. Our data support a role for...
Cells are first gated as viable GFP+ or mCherry+ cells, and normalized frequency of each progenitor compartment (HSC: CD34+CD38−CD90+CD45RA−, MPP: CD34+CD38−CD90−CD45RA−, and MLP: CD34+CD38−CD90−/+CD45RA+) within the gates is graphed. (D) VIS in CD14+ cells ...
However, these data demonstrate that somatic mutations are highly enriched in tumor infiltrating leukocytes compared to the overall hematopoietic compartment. TABLE 8 Deep sequencing of peripheral blood cells from breast cancer patients VAF tumor # VAF # VAF infiltrating variant MNC Coverage variant ...
Non-hematopoietic/endothelial SSEA-1pos cells defines the most primitive progenitors in the adult murine bone marrow mesenchymal compartment. Blood. 2007; 109 :1298–1306.Anjos-Afonso F, Bonnet D (2007) Nonhematopoietic/endothelial SSEA-1+ cells define the most primitive progenitors...
Minor histocompatibility antigens with expression restricted to the recipient hematopoietic compartment represent prospective immunological targets for graft-versus-leukemia therapy. It remains unclear, however, whether donor T cell recognition of these hematopoietically derived minor histocompatibility antigens ...
Mice lacking IL7Rα only on hematopoietic cells develop severe EAE, suggesting that IL7Rα expression in the nonhematopoietic compartment contributes to disease. Moreover, novel IL7Rα expression was identified on astrocytes and oligodendrocytes endogenous to the CNS. Chimeric mice that lack IL7Rα ...
By this method, a new compartment of multipotential progenitor cells has been identified, which give rise under differentiation conditions to progeny including osteoblasts, chondrocytes, myoblasts, adipocytes, and other non-hematopoietic cell types. Their use in cell and tissue-based engineering is ...