The iron-binding environment of the NHIO active site typically incorporates a '2-His-1-carboxylate' facial triad of amino acid side-chains, a motif that has emerged as a defining feature of the enzyme family. Towards the goal of biomimetic, iron-mediated C鈥揌 activation we have synthesized...
L. Structural insight into antibiotic fosfomycin biosynthesis by a mononuclear iron enzyme. Nature 437, 838–844 (2005). CAS PubMed Google Scholar Tarantino, G. & Hammond, C. Catalytic C(sp3)–F bond formation: recent achievements and pertaining challenges. Green Chem. 22, 5195–5209 (2020...
This discovery further prompted the conversion of the MT into a structurally/functionally unrelated new enzyme. Through stepwise mutagenesis and manipulation of coordination chemistry, MppJ was engineered to perform both Lewis acid-assisted hydration and/or O-methyltransfer reactions to g...
Now, a plant-derived non-haem iron enzyme, 1-aminocyclopropane-1-carboxylic acid oxidase, is repurposed and evolved to catalyse chemo- and enantioselective C(sp3)–H fluorination, forming a range of enantioenriched organofluorine products.Liu-Peng Zhao...
Endoperoxide formation by an α-ketoglutarate-dependent mononuclear non-haem iron enzyme. Nature, 2015, 527: 539-543.Yan, W.; Song, H.; Song, F.; Guo, Y.; Wu, C.-H.; Sae Her, A.; Pu, Y.; Wang, S.; Naowarojna, N.; Weitz, A.; et al. Endoperoxide formation by an α-...
Reported in this paper is the first example of a biomimetic iron complex, ([FeII(TPA)(NCMe)2]2+(TPA = tris(2-pyridylmethyl)amine), that catalyses the cis-dihydroxylation of an aromatic double bond, mimicking the action of the non-haem iron enzyme naphthalene dioxygenase and shedding light...
Enzyme Microb Technol 8:17-21. http://dx .doi.org/10.1016/0141-0229(86)90004-9.May, S. W., and A. G. Katopodis. 1986. Oxygenation of alcohol and sulphide substrates by a prototypical non-haem iron mono- oxygenase: catalysis and biotechnological potential. Enzyme Microb. Technol. 8:...
Oxygen-containing mononuclear iron species鈥攊ron(iii)鈥損eroxo, iron(iii)鈥揾ydroperoxo and iron(iv)鈥搊xo鈥攁re key intermediates in the catalytic activation of dioxygen by iron-containing metalloenzymes. It has been difficult to generate synthetic analogues of these three active iron鈥搊xy...
The enzyme was overproduced in S. lividans TK64 (up to 30000 times compared to S. aureofaciens ATCC 10762) and showed the same electrophoretic and immunological properties as the bromoperoxidase BPO-A2 purified from S. aureofaciens ATCC 10762. DNA sequence analysis revealed an open reading frame...
Redox-inactive metal ions that function as Lewis acids play pivotal roles in modulating the reactivity of oxygen-containing metal complexes and metalloenzymes, such as the oxygen-evolving complex in photosystem II and its small-molecule mimics. Here we report the synthesis and characterization of ...