The glucose consumption rates of the cells were determined by extracellular acidification rate (ECAR) thanks to the SeaHorse XFe24 instrument. In addition, gene expression profiles were determined by performing Total RNA sequencing with the Novaseq 6000 instrument. Finally, the expressions of GAPDH ,...
Glycolytic rate assay (B) and mitochondrial stress test (C) measuring extracellular acidification rate as a function of glycolytic activity and oxygen consumption as a function of mitochondrial respiration, respectively (n = 3, 4 technical replicates each, mean ± SEM). Mann–Whitney U: *p < ...
and Extracellular Acidification Rate(ECAR)assays.The target of miR-1294-5p was predicted by TargetScan and miRDB,which was further validated by flow cytometry analysis,RT-qPCR,western blotting,a dual-luciferase reporter assay,and RNA immunoprecipitation(RIP)assay.In the present study,it was found ...
Full size image While the loss of HK2 substantially abrogated extracellular acidification rate (ECAR) as a function of glycolytic activity in both HL60 and NB4 cell lines, loss of HK3 did not affect steady-state glycolytic activity (Fig.2B). At the same time, basal respiration was comparable ...
As a final product of anaerobic glycolysis, lactate is considered as one of the key catabolites responsible for media acidification and its toning towards yellow-orange shade [36]. In the light of this emerging evidence, we measured both glucose and lactate concentration in medium containing and ...