Non-specific binding (NSB) affects these two factors Substrate Ab1 Antigen Ab 2 Non Specific Binding : What is it? In addition to binding to receptors of interest, sec. antibody may also bind to other sites. Binding to the receptor of interest is called specific binding, while binding to ...
Non-specific binding When an antibody binds to unintended proteins (with highly similar epitopes) we speak of non-specificity. However, binding to closely related proteins may sometimes be unavoidable. Then the actual binding of the antibody may be specific, yet the antibody is deemed non-specific...
The current protocols for blocking background staining in immunohistochemistry are based on conflicting reports. Background staining is thought to occur as a result of either non-specific antibody (Ab) binding to endogenous Fc receptors (FcRs) or a combi
Immunostaining (non-fluorescent) secondary antibody dilution - chemical and physical properties, structure, melting point, boiling point, density, molecular formula, molecular weight, uses, prices, suppliers, and toxicity/safety/hazards/SDS information.
Product Name: Immunostaining (non-fluorescent) secondary antibody dilution Synonyms: CAS: MF: MW: 0 EINECS: Mol File: Mol File Information Error Report Your Email: Browse by Nationality Immunostaining (non-fluorescent) secondary antibody dilution Suppliers China suppliers Immunostaining (non-fluor...
Rabbit IgG (Sigma, St Louis, MO) was used as isotype control for detection of non-specific binding. Secondary antibody labeled with Alexaflour-488 was purchased from Life Technologies. Briefly, 5×106 cells infected with influenza A were washed in 2 ml PBS, pelleted by centrifugation, ...
RNA polymerase II core promoter proximal region sequence-specific DNA bindingtranscriptional repressor activity, RNA polymerase II core promoter proximal region sequence-specific bindingchromatin bindingprotein bindingSMAD binding 参与通路 negative regulation of transcription from RNA polymerase II promoterblastocyst...
(Invitrolon™ PVDF, Thermo Fisher Scientific, USA) and blocked in 5% nonfat dry milk for 2 hours. Membranes were then incubated with the appropriate dilutions of anti-mouse primary antibodies at 4 °C for 18 hours, followed by horseradish peroxidase-conjugated secondary antibody (Thermo ...
Plate wells were washed three times in TBS-T, and blocked again for 10 min prior to addition of 100 µl of diluted secondary antibody for 30 min. The plate was then washed and blocked as before prior to addition of 100 μl HRP chemi-luminescent substrate. Sample luminescence ...
The sections were then incubated with rabbit anti-GAP43 antibody (Huabio, Hangzhou, China) at a dilution of 1:1200 overnight at 4 °C. Afterwards, 1:2000 diluted goat anti-rabbit secondary antibody (Dako, Glostrup, Denmark) was applied for 30 min. The reaction products were visualized ...