previously been mutated as described above using sgRNAs targeting NEAT1 Region 2, NEAT1 Region 3 and negative control region, were resuspended in HBSS and mixed at ratio of 1:1 with Matrigel (Cat. # 356231; Corning, NY, USA) followed by subcutaneous injection into the flanks of the mice....
Two weeks later, the mice were anesthetized by intraperitoneal injection of 2% pentobarbital sodium. Subsequently, the knee joint cartilage was removed from each mouse and washed in ice-cold saline to remove the blood. After shearing and grinding, the knee joint cartilage was centrifuged at 3000...
Response to injection of 200 pA for 1 ms at x = 0.06, (A), and x = 0.04 cm, (B). This is not merely a three-dimensional view of Figure 9.8B, for here we see that the proximal stimulus did not elicit an action potential at the stimulation site (where the cable is only weakly...
At the age of 76 d, all pigs were anesthetized and euthanized with a jugular vein injection of 4% sodium pentobarbital solution (40 mg/kg body weight) after 12 h fasting. The animals were bled and opened immediately, blood samples (10 ml) were collected and centrifuged at 3000...
Injection when the tumor volume reached 100 mm3, whereas osimertinib (2 mg/kg) was initiated and repeated daily by oral gavage. Mouse body weight was recorded, and tumor volume was determined by caliper. Tumor volume was calculated following the formula of A × B2 × 0.5, ...
B82 was well tolerated with no obvious weight loss over the treatment period, suggesting that it is relatively nontoxic to mice (data not shown). Figure 4 B82 inhibited H460 xenograft tumor growth in athymic mice. One day after injected with H460 cells, once daily i.p. injection of B82...
About KEYTRUDA® (pembrolizumab) injection, 100 mg KEYTRUDA is an anti-programmed death receptor-1 (PD-1) therapy that works by increasing the ability of the body’s immune system to help detect and fight...
Injection of exosomes transfected with these increased miRNAs contributes to central obesity in lean mice by targeting PPARα.78 On the contrary, miR-26a from β cells and miR-690 from M2-polarized macrophages which are contained in exosomes can ameliorate IR induced by obesity.353,360 In ...
(Fig.3x). To determine the effect of GM4951 overexpression on liver triglycerides, we used hydrodynamic tail vein injection to deliver 3xFlag-Gm4951 expression plasmid to the liver. Two weeks after HFD, 3xFlag-Gm4951-injected mice had a decreased level of liver triglycerides compared with ...
water in 0.1% TFA at a flow rate of 0.5 ml/min. The samples injected each corresponded to an initial weight of tissue of hypothalamus 350 gr. (A), hypophysis 42 gr. (B), brain cortex 350 gr. (C). The arrows indicate the time of sample injection. Two minute fractions, except for ...