The lysate–resin mixture was added to a 65 g RediSep disposable sample load cartridge (Teledyne ISCO) and allowed to drain at room temperature. The protein-bound Ni-NTA agarose resin was then washed three times with 10 ml aliquots of wash buffer. The protein was eluted from the Ni-...
sRNA-seq and csRNA-seq libraries were then prepared using the NEB- Next Small RNA library kit (New England Biolabs) following the man- ufacturers protocol and sequenced SE75 in an Illumina NextSeq500. Total RNA was rRNA-depleted and library preparation was performed using the TruSeq Stranded...
disposable bioreactors (microbial vessel type). The vessels were filled with 150 mL M9 minimal salt media containing 10 g/L glucose as carbon source. Temperature was maintained at 30 ˚C throughout the fermentation process and agitation was set ...
disposable bioreactors (microbial vessel type). The vessels were filled with 150 mL M9 minimal salt media containing 10 g/L glucose as carbon source. Temperature was maintained at 30 ˚C throughout the fermentation process and agitation was set ...
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Tissue was transferred to a 15 mL conical tube, 1–2 mL of TRI Reagent was added, and the tissue was homogenized with a TissueRuptor (QIAGEN) and disposable probes. Samples were aliquoted in Eppendorf tubes (1 mL per tube) and phase separation was achieved with either 200 μL chloroform ...
All disposable supplies should be purchased sterilized and free of DNase and RNase. 5. Weigh glass tubes with lids. 6. In a biosafety cabinet (BSC), cut tissue using a sterile razor on aluminum foil that has been placed on dry ice. 7. In the BSC, place the tissue into a mortar pre...