It highlights Flp-In expression vectors [pcDNA5/CMV(-)/FRT/WT and pcDNA5/CMV(-)/FRT/MT1], where the luciferase gene is controlled by either the wild type or a G4-mutated RET promoter. The diagram visually represents these vectors' stable integration into the HEK 293 Flp-In cell line,...
Upon arrival in the cytoplasm, they are sorted into different organelles, such as mitochondria, or distributed in the cytoplasm. It is unclear how the ribosome binding property of cytoplasmic lncRNAs contributes to their functions. The structure of lncRNAs is very flexible, making them capable of i...
Selective degradation of host MicroRNAs by an intergenic HCMV noncoding RNA accelerates virus production Cell Host Microbe, 13 (2013), pp. 678-690 View PDFView articleView in ScopusGoogle Scholar Lee et al., 1988 S.I. Lee, S.C. Murthy, J.J. Trimble, R.C. Desrosiers, J.A. Steitz ...
The most important property of the filter chain is its order. When a request goes through pre-filters, the order is always determined from smallest to largest, from negative to positive. Post-filters in Zuul come after the proxied service processes it, and they are ordered from negative to ...
DNA plasmids encoding for C-terminally 3xFLAG-tagged Gai3 (ref. 25) or an empty plasmid (p3xFLAG-CMV-14) were transfected using the calcium phosphate method in 10 cm dishes. The DNA amount for the Gai3 N269D plasmid was threefold (12 mg) of that for Gai3 WT (4 mg) to equalize ...
CMV: Cytomegalovirus CpG: 5’-C-phosphate-G-3’ CRISPR: Clustered regularly interspaced short palindromic repeats CXCR4: C-X-C chemokine receptor type 4 DEX: Dexamethasone DNA: Deoxyribonucleic acid DOPE: 1,2-Dioleoyl-sn-glycero-3-phosphoethanolamine ...
2. FIG. 2 depicts the results of a second messenger cAMP accumulation assay providing comparative results for constitutive signaling of endogenous FSHR (“WT”), non-endogenous version of FSHR (“L460R”) and a control vector (“CMV”). This data further evidences that the L460R version of ...
4.The method of claim 2, wherein(i) the first and/or second detectable marker peptide is a fluorescence protein, and the constitutive promoter of the expression cassette is selected from GAGGS, CMV, PGK, and TK; and/or(ii) the first and/or second detectable marker is a protein. ...
For expression, two common human alleles were inserted into the transient expression vector pcDNA5/FRT, which has a human CMV promoter and a bovine growth hormone (bgh) polyadenylation signal, FIG. 3.Working Plasmid ConstructionsThe secretion signal sequence and codons 1-276 of mature HUMMHCREP ...
Should homologous plasma fibronectin be used, lots prepared from different donors would have to be tested for atypical antibodies, hepatitis B (HBV), hepatitis C (HCV), human immunodeficiency virus (HIV), human T-cell lymphotrophic virus (HTLV), cytomegalovirus (CMV) and syphilis. These tests ...