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IECs were stained and sorted in 1x PBS with 5% FBS and 2mM EDTA to reduce cell clumping on ice in 1.5 ml microcentrifuge tubes. For intracellular staining, cells were fixed and permeabilized using BD Cytofix/Cytoperm kit (BD Bioscience). Samples were acquired on an LSRII flow cytometer (BD...