Objectives This randomized, triple-blind, crossover clinical trial aimed to evaluate the efficacy, onset, length of pulp and soft tissue anesthesia, and pain during injection of 2% buffered articaine and 4% non-buffered articaine solutions. Methods Each volunteer received two maxillary supraperiosteal...
After that, organs were kept in 10% neutral buffered formalin (NBF) followed by dehydration in 10%, 20% and 30% sucrose; 5 μm lung sections were prepared. Characterization of H1N1 virus labelled with DiD Fӧrster resonant energy transfer assay was applied using PE-Alexa 610 (Q610) ...
in 2.0% 2,3,5-triphenyltetrazolium chloride (Solarbio, Beijing, China) at 37 °C for 15 min for measurement of the infarct area. The staining was stopped by ice-cold sterile saline and the slices were fixed in 4% neutral buffered formaldehyde for another 5 min. Both sides of each...
(after // wrapping the channel in a throttle you should no longer call any of the // channel-methods directly) AMQP::Throttle throttle(connection, 20); // publish way more messages than RabbitMQ can handle (the Throttle class // will make sure that messages are buffered inside your ...
C for growth to OD 600 ¼ 0.6. Protein expression was initiated by addition of Isopropyl-b-D-thiogalactopyranoside (IPTG) to a final concen- tration of 1 mM. Induction continued for 2 h before the cells were pelleted in a centrifuge, washed with phosphate-buffered ...
spinal cords indicate that, regardless of the sex, androgens up-regulate genes related to neuronal function integrity and myelin production. Depending on the sex, androgens down-regulate genes related to the immune system in females and lipid catabolism in males. Thus, androgens are required for ...
'buffered_stdio': 1, 'bytes_warning': 0, 'check_hash_pycs_mode': 'default', 'configure_c_stdio': 0, 'dev_mode': 0, 'dump_refs': 0, 'exec_prefix': '/usr/local',-'executable': '/home/alexei/\udcd0\udc97\udcd0\udcb0\udcd0\udcb3\udcd1\udc80\udcd1\udc83\udcd0\udcb7\udc...
Also, remove fcntl_mode as the blocking mode is no longer buffered. It is now a product of the timeout. Add prototype for a wrapper vio_timeout() function, which invokes the underlying timeout method of the VIO. Add to VIO two member variables to hold the read and write timeout ...
Briefly, aortic valve leaflets were isolated and washed with phosphate-buffered saline (PBS) containing 1% penicillin/streptomycin for 3 times, and then were cut into small pieces with sterile surgical scissors, followed by digestion in 2 mg/mL type I collagenase for 2–5 h at 37 °C. Show...
8g). While the positive control (buffered EDTA intracardiac injection for 5 min) resulted in robust deposition of fluorescent beads in between cells, no accumulation of fluorescent beads was found in any of the other groups (Extended Data Fig. 8g). Thus, MacAIR cells do not play a role ...