Even a well-prepared non-binding LOI can be enforceable if the parties negotiating don't understand the potential results of their behavior. While this type of document is almost always meant to be non-binding, it's common to include some binding provisions related to confidentiality, marketing...
Nonwoven fabric is typically manufactured by layering medium sized fibers (4–7cm) in the form of a sheet or a web and then binding them either mechanically, thermally or with an adhesive. From:Biocatalysis and Agricultural Biotechnology,2014 ...
Surface staining of mouse cells was performed after 15 min pre-incubation with anti-mouse CD16/CD32 Ab (clone 2.4G2; BD Biosciences) to block Fcγ receptor binding, with panels of appropriately diluted fluorochrome-conjugated Abs (from BD Biosciences, eBioscience or Invitrogen) against the ...
Since the templates for the TA complex were available (2p5t, 1gvn), restraints were derived based on known interfacial residues in the template that are conserved in the alignments (Fig. 2). Both the programs predicted at least one binding mode with hydrogen bond between Arg116 in PezT...
Apart from specific interactions within active sites, this could be explained by the fact that HDAC2 displays the smallest binding pocket among the isoforms considered in this study (Table S7)51. This may prevent the accommoda- tion of compounds possessing bulky moieties12,34. In this study, ...
Cells were stained with Alexa Fluor ®647 Annexin V (BioLegend, San Diego, CA, USA) and propidium iodide (ThermoFisher Scientific) in annexin V binding buffer (10 mM HEPES, 140 mM NaCl, 2.5 mM CaCl2) for 15 min and analysed using the BD LSRFortessa™ X-20 (BD Biosciences). ...
The binding of Polo is required for both the recruitment of Asl to the daughter centriole and for centriole conversion, the process whereby the daughter centriole is converted to a mother centriole capable of duplicating and recruiting PCM to organize a centrosome [40]. Conversion requires the ...
Moreover, in vivo concomitant binding of STAT3 and SP1 defines a subclass of genes belonging to the non-canonical WNT and the Interleukin (IL)-6/STAT3 pathways and contributing to breast cancer aggressiveness, suggesting the relevance of developing novel targeted therapies combining inhibitors of the...
LINE-1 contains two open reading frames, L1-ORF1 and L1-ORF2, that encode for L1-ORF1p (RNA binding pro- tein) and L1-ORF2p (reverse transcriptase and endonuclease) proteins, respectively. These two proteins cooperate to introduce the retrotransposon sequence into new chromatin target sites ...
As a template, either the starting DNA library in the first selection round was used or the sorted DNA library cloned into the pRDVsm vector in the next rounds. The resulted amplicon contained a T7 promoter, 5 stem-loop, ribosome binding site (RBS), start codon, N-terminal Strep-tag II...