It was John Maynard Keynes who once famously alluded to the thin dividing line between Fair & Foul in a Nation’s Economics. Like most of his quotable quotes, this too is applicable to most of our problems which have eluded solutions despite well-meaning policy formulation & budgetary allocati...
An embodiment of designing the HDR sequence to reduce specific binding of the DNA-binding member comprises placing a mismatch in the HDR template sequence as aligned with the endogenous chromosomal DNA. The mismatch may include one or more of: an insertion, a deletion, a substitution, an ...