(chilled to 4 °C). The SDC buffer contained 1% SDC, 10 mM TCEP, 40 mM CAA, 75 mM Tris-HCl at pH = 8.5 and its pH was adjusted to 8.5 with 1 N NaOH. The urea buffer contained 8 M urea, 1 mM EDTA, 1 mM CAA, 150 mM NaCl, 50 mM Tris-HCl pH...
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