Patterns of emergent resistance-associated mutations after initiation of non-nucleoside reverse-transcriptase inhibitor-containing antiretroviral regimens in Taiwan: a multicenter cohort study Chien-Yu Cheng, Mao-Song Tsai, Chia-Jui Yang, Shu-Hsing Cheng, Hsin-Yun Sun, Shu-Fang Chang, Li-Hsin Su, ...
9–13 These include signal recognition particle assembly, small RNA modification, RNA editing, telomerase maturation, nuclear export, cell cycle control, and stress sensing. Some telomere-related proteins such as telomerase and TRF1 have been reported to localize in the nucleolus or to interact with...
Dynamic changes of throat swabs RNA and serum antibodies for SARS-CoV-2 and their diagnostic performances in patients with COVID-19 Xueping Qiu, Yang Xiang, Jianbin Sun, Xinrui Wang, Guoqiang Chen, Xianqun Xu, Shengjun Liao, Na Yang, Siwei Li, Gang Yang, Yueting Tang, Junli Fan, Wen ...
RT-PCR. Total RNA was extracted from cells with RNA- zolB (Biotecx Laboratories; Houston, TX, U.S.A.) according to the manufacturer's instructions. First-strand cDNA synthesis of total RNA was performed using SuperScript RNase HϪ Reverse Transcriptase (GibcoBRL) and random hexamer prim- ...
微小RNA (miRNA) 充当辅助表观遗传系统,可以调节包括乙肝相关肝细胞癌 (HBV-HCC) 在内的所有癌症途径中的基因组表达。miRNA 调控癌症发病机制的分子机制为研究人员提供了一种新方法,人们普遍希望这种方法能够转化为基于 miRNA 的药物和诊断方法。然而,三十年后,基于 miRNA 的 HCC 诊断和治疗剂仍处于开发阶段 (WIP...
(ex 485 nm, em 538 nm, arbitrary units, calculated as percent in relation to the control (high glucose medium: HG), mean ± SD,n= 5). Cells incubated with a mixture of cytokines (Cytomix) were used as a negative control (***p< 0.001). (B) XTT assay. The XTT assay was ...
Figure 6. Volcano plots (a) of RNA-seq data for an A vs B pairwise comparison; (b) the top 30 differentially-regulated TF families were identified among all the genes. A: NO2 control. B: NO2 stressed. q-value, <0.05; fold change, >2. Table 3. Annotation of A vs B DEGs in...
Reverse transcriptase Purchase SuperScript IV Vilo Master Mix separately and use before qPCR Assay recommendations Sequence detection primers Request a sample Note: when you need up to 72 hours of benchtop stability, choose PowerUp SYBR Green Master Mix in combination ...
Sequences predicted to form a stem-loop structure are marked with divergent thick arrows. B, reverse transcriptase-PCR amplifications to identify introns in the 5′-unstranslated region of crgA. Total RNA isolated from the wild type strain R7B grown in the dark for 2 days, illuminated for ...
RNA was quantified by a Nanodrop 1000 spectrophotometer and stored at −80 °C cDNA was synthesised with an iScript cDNA synthesis kit (Bio-Rad). The reaction mix consisted of: 2 µL of 5× reaction buffer, 0.5 µL of iScript reverse transcriptase, 200 ng of RNA, oligo...