(D6M5X) monoclonal antibodies (CST, #23214, immunofluorescence 1:200); Mouse anti-FLAG (M2) monoclonal antibodies (Sigma, #F1804, Western 1:1000); Rabbit anti-TIM23 polyclonal antibodies (Proteintech, #11123-1-AP, Western 1:1000); Rabbit anti-p62 polyclonal antibodies (Proteintech, #18420...
The paraffin sections were stained with either monoclonal anti-NOLC1 (A, C–E) or the control IgG (B, F). The NOLC1 immunoreactivity was seen clearly in the nucleoli of the tumor cell (arrows) in the tumor nests, but not in the stromal cells or in the normal squamous metaplastic ...
The electrophoresis was positive for high drug concentrations in the three monoclonal antibody groups, being the signals weaker with decreasing concentrations. Same result was obtained with immunofixation, noting a band corresponding to a kappa G immunoglobulin which migrates closer to the cathode for ...
Using this construct, we now carried out AP-MS with a highly specific anti-FLAG monoclonal antibody to investigate NOA36 interactors in a non-biased way. Thus, although several proteomics studies have already identified possible human NOA36 interactor-proteins (Q9Y3S2 UNIPROT entry), we disclosed...
precipitation was detected by western blot.cLcPIP1 physically interacted with PlPeL1 and PlPeL1-like in vitro. GST-PlPeL1-, GST-PlPeL1-like-, or GST- bound beads were incubated with bacteria lysate containing His-LcPIP1 or His-NbPIP1. Co-precipitation was detected by western blot. Red...
A gastric pouch of approximately 20 mL is insuffciencies that must be medically managed long- created by infating the band using a subcutaneous term. This band can be adjusted by the physician at ity of the stomach to store food, thereby inducing offce visits to accommodate the needs of ...
Live synaptotagmin 1 labeling: We performed labeling of synaptotagmin 1 with a mouse monoclonal purified IgG conjugated with Chromeo 488 (105 311 CR1, Synaptic Systems) targeting the lumenal domain of synaptotagmin 1. The IgGs were added to the culture medium of cells (0.3 μg mL−1)...
Results We confirmed depletion of spectrin and the presence of band 3, CD47 and glycophorins in vesicles released from normal red cells. Confocal microscopy demonstrated the coating of Rh(D+) vesicles with human Mab anti-D. Other membrane molecules detected on the vesicles were Duffy glycoprotein...
Moreover, when non-target siRNA-treated U-1810 cells were irradiated, only a faint band of cleaved PARP was detected at 48 h post IR but with an increase observed at 120 h post IR (Figure 4a). In contrast, silencing of Ephrin B3 in combination with IR initiated a strong PARP cleavage...
The blots were incubated with anti-mouse Ryr3 monoclonal antibody (1/250) or anti-13-actin (1:5000) overnight at 4° C. in 1.5% BSA/1.5% milk/PBS buffer. LC3, CAMK IV and calmodulin were separated on 16% Tris-glycine gel (Novex) and detected by anti-LC3 (1:1000), anti-CAMK ...