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It may be that residual HAD5 activity, below the limit of detection by immunoblot analysis, is present after knockdown and sufficient for generating detectable GPI precursors and subsequent tethering of some GPI-APs. Alternatively, P. falciparum expresses two annotated PGMs (PF3D7_1012500 and PF3D...
Some techniques bring a paradoxical approach to the escalation sequence by pushing a request to or beyond its acceptable limit and then backing off. In the door-in-the-face (sometimes called the reject-then-compromise) procedure, the persuader begins with a large request they expect will be rej...