STD方法的一个主要优点是:这种方法可以与很多NMR脉冲序列结合,产生一系列的饱和转移差谱NMR实验,例如STD-TOCSY,STD-HSQC,等等。 饱和转移差谱(saturationtransferdifferenc,STD)实验原理如Fig 1所示:用圆形和三角形分别表示与蛋白有、无结合的配体,蓝色不规则形状代表蛋白大分子,分别做参照实验(off-resonance)(左图)和...
STD-NMR theSaturationTransferDifference(STD)STD-NMRstudiesoftwoacceptorsubstratesofGlfT2,agalactofuranosyltransferasefromMycobacteriumtuberculosis:Epitopemappingstudies STD-DOSY:AnewNMRmethodtoanalyzemulti-componentenzyme/substratesystems Fig.1.PulsesequencefortheSTD-DOSYexperiment(p1correspondstoa90,p2toa180highpower...
STD-NMR STD-NMRstudiesoftwoacceptorsubstratesofGlfT2,agalactofuranosyltransferasefromMycobacteriumtuberculosis:Epitopemappingstudies STD-DOSY:AnewNMRmethodtoanalyzemulti-componentenzyme/substratesystems theSaturationTransferDifference(STD) Fig.1.PulsesequencefortheSTD-DOSYexperiment(p1correspondstoa90,p2to ...
STD-NMR theSaturationTransferDifference(STD)STD-NMRstudiesoftwoacceptorsubstratesofGlfT2,agalactofuranosyltransferasefromMycobacteriumtuberculosis:Epitopemappingstudies STD-DOSY:AnewNMRmethodtoanalyzemulti-componentenzyme/substratesystems Fig.1.PulsesequencefortheSTD-DOSYexperiment(p1correspondstoa90,p2toa180highpower...
Saturation transfer difference NMR (STD NMR) spectroscopy is one of the most powerful NMR techniques for detection and characterization of transient (fast)
NMR STD tr-Noesy fast effective screen screening plant extractJimenezBarbero, J
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Saturation transfer difference (STD) NMR-based binding studies were performed to investigate the binding potential of two bisphenol representatives, namely, bisphenol B (BPB) and bisphenol E (BPE), toward human serum albumin (HSA). The relative STD (%) suggested that BPB and BPE show similar ...